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An investigation on the c-MYC, AXIN1, and COL11A1 gene expression in colorectal cancer
- Source :
- Biotechnology and applied biochemistryREFERENCES. 69(4)
- Publication Year :
- 2021
-
Abstract
- The high incidence rate of CRC demands early diagnosis of the disease and readiness of diagnostic biomarker. In present study, we have investigated c-MYC, AXIN1, and COL11A1 expression levels in course of CRC progression and their correlation with demographics and clinical risk factors. Fifty-five tumors and 41 normal tissues were obtained from Tumor Bank of Iran, total RNA was extracted, cDNA was synthesized, and RT-qPCR was performed. Results were analyzed using Rest 2009 and SPSS software. Analysis at mRNA level showed upregulation of the two genes; c-MYC with a p-value of 0.001 and COL11A1 with an observed p-value of 0.02, while a p-value of 0.04 indicated AXIN1 downregulation. The observed overexpression of COL11A1 in stage 0 compared to other stages of CRC asserts importance of this gene in CRC prognosis. Moreover, statistical analysis confirms a significant correlation between expression of these genes and several clinical risk factors of CRC. Our study supports the importance of the studied genes and provides further information regarding the molecular mechanism of CRC. Further studies on these genes could elucidate their pivotal role for both early detection and/or diagnosis of CRC in addition to have important biomarkers for CRC management available.
- Subjects :
- Oncology
medicine.medical_specialty
Colorectal cancer
Biomedical Engineering
Gene Expression
Bioengineering
Disease
Collagen Type XI
Applied Microbiology and Biotechnology
Proto-Oncogene Proteins c-myc
Downregulation and upregulation
Axin Protein
Complementary DNA
Internal medicine
Drug Discovery
Gene expression
AXIN1
medicine
Biomarkers, Tumor
Humans
RNA, Messenger
Stage (cooking)
Gene
business.industry
Process Chemistry and Technology
General Medicine
medicine.disease
Prognosis
digestive system diseases
Up-Regulation
Gene Expression Regulation, Neoplastic
Molecular Medicine
business
Colorectal Neoplasms
Biotechnology
Subjects
Details
- ISSN :
- 14708744
- Volume :
- 69
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biotechnology and applied biochemistryREFERENCES
- Accession number :
- edsair.doi.dedup.....c2e10dbbf3af589ed90dde403a2c1e91