Back to Search
Start Over
SMBA1, a Bax Activator, Induces Cell Cycle Arrest and Apoptosis in Malignant Glioma Cells
- Source :
- Pharmacology. 105(3-4)
- Publication Year :
- 2018
-
Abstract
- SMBA1 (small-molecule Bax agonists 1), a small molecular activator of Bax, is a potential anti-tumour agent. In the present study, we investigated the biological effects of SMBA1 on glioblastoma (GBM) cells. SMBA1 reduced the viabilities of U87MG, U251 and T98G cells in a time- and dose-dependent manner. Moreover, treatment with SMBA1 induced cell cycle arrest at the G2/M phase transition, accompanied by the downregulation of Cdc25c and cyclin B1 and the upregulation of p21. SMBA1 also induced apoptosis of GBM cells in a dose-dependent manner. Mechanistically, SMBA1 induced apoptosis via the intrinsic pathway. Silencing of Bax or ectopic expression of Bcl-2 significantly inhibited SMBA1-induced apoptosis. Moreover, SMBA1 inhibited the growth of U87MG xenograft tumours in vivo. Overall, SMBA1 shows anti-proliferative effects against GBM cells through activation of the intrinsic apoptosis pathway.
- Subjects :
- Male
Cell cycle checkpoint
Time Factors
Mice, Nude
Antineoplastic Agents
Apoptosis
Mice
Downregulation and upregulation
Glioma
Cell Line, Tumor
medicine
Animals
Humans
Cyclin B1
bcl-2-Associated X Protein
Pharmacology
Mice, Inbred BALB C
Dose-Response Relationship, Drug
Chemistry
Activator (genetics)
Brain Neoplasms
Intrinsic apoptosis
General Medicine
Cell Cycle Checkpoints
medicine.disease
Xenograft Model Antitumor Assays
Cancer research
Ectopic expression
Subjects
Details
- ISSN :
- 14230313
- Volume :
- 105
- Issue :
- 3-4
- Database :
- OpenAIRE
- Journal :
- Pharmacology
- Accession number :
- edsair.doi.dedup.....c2ce9279be5bb3c23d8cab9f6f87289f