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Expression and chromosomal localization of a lymphocyte K+ channel gene

Expression and chromosomal localization of a lymphocyte K+ channel gene

Authors :
Stephan Grissmer
Alan L. Goldin
George A. Gutman
Michael D. Cahalan
John J. Wasmuth
Brent A. Dethlefs
K G Chandy
Source :
Proceedings of the National Academy of Sciences of the United States of America. 87(23)
Publication Year :
1990

Abstract

We recently isolated a family of three closely related mouse K+ channel genes (MK1, MK2, and MK3) with coding regions contained in single uninterrupted exons. Here we have used patch-clamp recordings from Xenopus oocytes injected with mRNA to show that MK3 encodes a channel with biophysical and pharmacological properties indistinguishable from those of voltage-gated type n K+ channels in T cells. In addition, we used the polymerase chain reaction to demonstrate the presence of MK3 mRNA in T cells. These data suggest that MK3 may encode the T-cell voltage-gated type n K+ channel. We also show that MK3 and MK2 are localized on human chromosomes 13 and 12, respectively.

Details

ISSN :
00278424
Volume :
87
Issue :
23
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences of the United States of America
Accession number :
edsair.doi.dedup.....c2ce38ff0a3647e50686ed8d83f6002a