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Expression and chromosomal localization of a lymphocyte K+ channel gene
Expression and chromosomal localization of a lymphocyte K+ channel gene
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 87(23)
- Publication Year :
- 1990
-
Abstract
- We recently isolated a family of three closely related mouse K+ channel genes (MK1, MK2, and MK3) with coding regions contained in single uninterrupted exons. Here we have used patch-clamp recordings from Xenopus oocytes injected with mRNA to show that MK3 encodes a channel with biophysical and pharmacological properties indistinguishable from those of voltage-gated type n K+ channels in T cells. In addition, we used the polymerase chain reaction to demonstrate the presence of MK3 mRNA in T cells. These data suggest that MK3 may encode the T-cell voltage-gated type n K+ channel. We also show that MK3 and MK2 are localized on human chromosomes 13 and 12, respectively.
- Subjects :
- Potassium Channels
T-Lymphocytes
Xenopus
Molecular Sequence Data
Membrane Potentials
Exon
Gene expression
Coding region
Animals
Patch clamp
Gene
Messenger RNA
Multidisciplinary
biology
Base Sequence
Chromosome Mapping
biology.organism_classification
Molecular biology
Potassium channel
Rats
Electrophysiology
Multigene Family
Oocytes
Female
Oligonucleotide Probes
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 87
- Issue :
- 23
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....c2ce38ff0a3647e50686ed8d83f6002a