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CEACAMs interaction with Helicobacter pylori HopQ supports the type 4 secretion system-dependent activation of non-canonical NF-κB
- Source :
- International Journal of Medical Microbiology, Vol 310, Iss 6, Pp 151444-(2020)
- Publication Year :
- 2019
-
Abstract
- Helicobacter pylori infection represents a major risk factor for the development of gastric diseases and gastric cancer. The capability of H. pylori to inject the virulence factor cytotoxin-associated gene A (CagA) depends on a type IV secretion system (T4SS) encoded by the cag pathogenicity island (cagPAI). Further, infection by H. pylori activates the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in a T4SS-dependent manner but CagA-independent manner. Here we investigated the role of host cell receptors carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) and the bacterial adhesin HopQ in the activation of non-canonical NF-κB and CagA translocation into gastric epithelial cells. AGS cells express six of twelve CEACAMs found in humans. In HeLa cells, only CEACAM19 is expressed. We showed that deletion of hopQ attenuates the activation of non-canonical NF-κB only in AGS but not in HeLa cells. CagA translocation was in both cell lines affected by HopQ depletion, although to a much lesser extent in HeLa cells. Moreover, we observed a possible redundancy between the three HopQ-binding CEACAMs 1, 5 and 6 and their capacity to support non-canonical NF-κB activation. Our results illustrate that the interaction between HopQ and CEACAMs could promote the efficiency of the T4SS.
- Subjects :
- Microbiology (medical)
RelA
RelB
Biology
Microbiology
Virulence factor
p100
Helicobacter Infections
HeLa
Type IV Secretion Systems
03 medical and health sciences
Other systems of medicine
Bacterial Proteins
Antigens, Neoplasm
Cytotoxin-associated gene A (CagA)
CagA
Outer membrane protein (OMP)
Humans
Secretion
030304 developmental biology
0303 health sciences
Antigens, Bacterial
Helicobacter pylori
030306 microbiology
Cell adhesion molecule
NF-kappa B
Epithelial Cells
General Medicine
NF-κB-inducing kinase (NIK)
biology.organism_classification
Molecular biology
QR1-502
Bacterial adhesin
Infectious Diseases
Cell culture
Cell Adhesion Molecules
RZ201-999
HeLa Cells
Subjects
Details
- ISSN :
- 16180607
- Volume :
- 310
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- International journal of medical microbiology : IJMM
- Accession number :
- edsair.doi.dedup.....c2cae3f9048d5434ab9ed1857bfe1990