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Molecular analysis of a t(11;14)(q23;q11) from a patient with null-cell acute lymphoblastic leukemia
- Source :
- Genes, Chromosomes and Cancer. 7:38-46
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- /1p;&-3qChromosome 11, band q23, is the frequent site of recurring cytogenetic rearrangements in human leukemia. We have cloned and sequenced the breakpoint junctions from a patient who had null-cell acute lymphoblastic leukemia (ALL) with a t(11;14)(q23;q11). The chromosome 14 breakpoints occurred within the TCRD locus, close to two diversity segments. The chromosome 11 breakpoint occurred between two head-to-head heptamer sequences, and junctional diversity was evident at both derivative junctions, suggesting involvement of the V(D)J recombinase. The TCRA/D locus on the normal chromosome 14 had undergone a Vδ2-Dδ3-ΨJα joining. Two phage clones with this VDJ rearrangement were isolated; one of these contained an intra-Jα region deletion. Two clones with the derivative 11 junction were isolated; one of these had a similar, but not identical, deletion. A heptamer-nonamer recognition sequence (located ∼70 kb 5′ to Cα), not associated with a TCR gene coding segment, was found in the immediate vicinity of both 5′ breakpoints. We have designated this sequence 5′del for 5′ deleting element. An intra-Jα region deletion involving this heptamer-nonamer was previously identified in the leukemia cells recovered from a patient who had T-cell ALL. Fifty kilobases of DNA on 11q23 surrounding the breakpoint were cloned and analyzed. No CpG islands or conserved sequences were identified within this region. Fluorescence in situ hybridization analysis showed that this 11q23 breakpoint mapped distal to the MLL gene associated with the recurring breakpoints in the 4;11, 9;11, and 11;19 translocations, distal to the RCK gene associated with an 11;14 translocation, and proximal to the ETSI gene, which is located at 11q24. © 1993 Wiley-Liss, Inc.
- Subjects :
- Adult
Cancer Research
Receptors, Antigen, T-Cell, alpha-beta
Molecular Sequence Data
Restriction Mapping
Chromosomal translocation
Locus (genetics)
Biology
Translocation, Genetic
Deoxyribonuclease EcoRI
Conserved sequence
Genetics
medicine
Recombinase
Humans
Cloning, Molecular
Gene
Gene Library
Sequence Deletion
Chromosomes, Human, Pair 14
Gene Rearrangement
Base Sequence
medicine.diagnostic_test
Chromosomes, Human, Pair 11
Breakpoint
Chromosome Mapping
Receptors, Antigen, T-Cell, gamma-delta
DNA, Neoplasm
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Molecular biology
Junctional diversity
Chromosome Banding
Immunoglobulin Joining Region
Female
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 10982264 and 10452257
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Genes, Chromosomes and Cancer
- Accession number :
- edsair.doi.dedup.....c2b8aefbc44edf23f46905c4b489f756