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Expression of Angiogenesis Regulatory Proteins and Epithelial-Mesenchymal Transition Factors in Platelets of the Breast Cancer Patients
- Source :
- The Scientific World Journal, The Scientific World Journal, Vol 2014 (2014)
- Publication Year :
- 2014
- Publisher :
- Hindawi Publishing Corporation, 2014.
-
Abstract
- Platelets play a role in tumor angiogenesis and growth and are the main transporters of several angiogenesis regulators. Here, we aimed to determine the levels of angiogenesis regulators and epithelial-mesenchymal transition factors sequestered by circulating platelets in breast cancer patients and age-matched healthy controls. Platelet pellets (PP) and platelet-poor plasma (PPP) were collected by routine protocols. Vascular endothelial growth factor (VEGF), platelet-derived growth factor BB (PDGF-BB), thrombospondin-1 (TSP-1), platelet factor 4 (PF4), and transforming growth factor-β1 (TGF-β1) were measured by enzyme-linked immunosorbent assay. Angiogenesis-associated expression of VEGF (2.1 pg/106platelets versus 0.9 pg/106platelets,P < 0.001), PF4 (21.2 ng/106platelets versus 10.2 ng/106platelets,P < 0.001), PDGF-BB (42.9 pg/106platelets versus 19.1 pg/106platelets,P < 0.001), and TGF-β1 (15.3 ng/106platelets versus 4.3 ng/106platelets,P < 0.001) differed in the PP samples of cancer and control subjects. In addition, protein concentrations were associated with clinical characteristics(P<0.05). Circulating platelets in breast cancer sequester higher levels of PF4, VEGF, PDGF-BB, and TGF-β1, suggesting a possible target for early diagnosis. VEGF, PDGF, and TGF-β1 concentrations in platelets may be associated with prognosis.
- Subjects :
- Adult
Blood Platelets
Vascular Endothelial Growth Factor A
medicine.medical_specialty
Epithelial-Mesenchymal Transition
Article Subject
Angiogenesis
medicine.medical_treatment
Becaplermin
lcsh:Medicine
Gene Expression
Breast Neoplasms
Biology
Platelet Factor 4
lcsh:Technology
General Biochemistry, Genetics and Molecular Biology
Neovascularization
Thrombospondin 1
Transforming Growth Factor beta1
chemistry.chemical_compound
Internal medicine
medicine
Biomarkers, Tumor
Humans
Platelet
lcsh:Science
General Environmental Science
Aged
Neoplasm Staging
Neovascularization, Pathologic
lcsh:T
Growth factor
lcsh:R
General Medicine
Proto-Oncogene Proteins c-sis
Middle Aged
Vascular endothelial growth factor
Vascular endothelial growth factor A
Endocrinology
chemistry
Case-Control Studies
biology.protein
lcsh:Q
Female
medicine.symptom
Platelet factor 4
Platelet-derived growth factor receptor
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 1537744X and 23566140
- Volume :
- 2014
- Database :
- OpenAIRE
- Journal :
- The Scientific World Journal
- Accession number :
- edsair.doi.dedup.....c2a4f6ea8b4d9d8f2f77aab5e5d91f1e