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MicroRNA-425 controls lipogenesis and lipolysis in adipocytes
- Source :
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1864:744-755
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- An increasing number of studies have demonstrated that some microRNAs participate in the regulation of growth and development of adipocytes. The present study shows that microRNA-425-5p (miR-425) is a novel strong regulator of adipogenesis and adipolysis in adipocytes. Forced expression of miR-425 in mice promoted body fat accumulation and the development of obesity due to high-fat intake, whereas silencing miR-425 prevented mice from being obese. Mechanically, the expression of miR-425 is controlled by PPARĪ³ during the adipogenesis process in adipocytes. MiR-425 overexpression resulted in a reduction in the proliferation of 3t3-L1 pre-adipocytes but significantly accelerated cellular adipogenic differentiation. Mapk14, a negative regulator of adipogenesis, was predicted and confirmed as a real target gene of miR-425. Moreover, knocking down miR-425 remarkably intensified intracellular lipolysis and promoted lipid oxidation, which is related to the activation of AMPK, a monitor for intracellular energy balance. MiR-425 activated AMPK not only by decreasing cellular ATP concentrations but also by targeting the gene of Cab39, which is an upstream co-activator of AMPK. The findings of the present study suggest that miR-425 could control adipogenesis and adipolysis in adipocytes by simultaneously triggering multidirectional targets.
- Subjects :
- 0301 basic medicine
Lipolysis
Mitogen-Activated Protein Kinase 14
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Lipid oxidation
3T3-L1 Cells
Adipocyte
microRNA
Adipocytes
Animals
Gene silencing
Obesity
Molecular Biology
Adipogenesis
Chemistry
Lipogenesis
AMPK
Cell Biology
Cell biology
PPAR gamma
MicroRNAs
030104 developmental biology
Gene Expression Regulation
030220 oncology & carcinogenesis
Subjects
Details
- ISSN :
- 13881981
- Volume :
- 1864
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
- Accession number :
- edsair.doi.dedup.....c2872a18307ddcbe5c5237802ae2431f