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Genome-Wide Expression Screening Discloses Long Noncoding RNAs Involved in Thyroid Carcinogenesis
- Source :
- The Journal of Clinical Endocrinology & Metabolism. 101:4005-4013
- Publication Year :
- 2016
- Publisher :
- The Endocrine Society, 2016.
-
Abstract
- Long noncoding RNAs (lncRNAs) regulate pathological processes, yet their potential roles in papillary thyroid carcinoma (PTC) are poorly understood.To profile transcriptionally dysregulated lncRNAs in PTC and identify lncRNAs associated with clinicopathological characteristics.We performed RNA sequencing of 12 paired PTC tumors and matched noncancerous tissues and correlated the expression of lncRNAs with clinical parameters. The 2 most significantly dysregulated lncRNAs were studied in an Ohio PTC cohort (n = 109) and in PTC data (n = 497) from The Cancer Genome Atlas.A combination of laboratory-based studies and computational analysis using clinical data and samples and a publically available database.Correlation between expression values and clinical parameters.We identified 218 lncRNAs showing differential expression in PTC (fold change ≥ 2.0, P.01). Significant correlation was observed between the expression of 2 lncRNAs (XLOC_051122 and XLOC_006074) and 1) lymph node metastasis (N stage) and 2) BRAF(V600E) mutation. Among patients with wild-type BRAF, the expression of these 2 lncRNAs showed significantly higher levels in the patients with lymph node metastasis. In silico analysis of these lncRNAs pinpointed cell movement and cellular growth and proliferation as targeted functions.Comprehensive expression screening identified 2 novel lncRNAs associated with risk factors of adverse prognosis in PTC patients. These lncRNAs may be novel players in PTC carcinogenesis.
- Subjects :
- 0301 basic medicine
endocrine system diseases
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Thyroid Gland
Bioinformatics
medicine.disease_cause
Biochemistry
Cohort Studies
0302 clinical medicine
Endocrinology
Gene expression
RNA, Neoplasm
Thyroid
Outcome measures
Prognosis
Up-Regulation
medicine.anatomical_structure
Thyroid Cancer, Papillary
Lymphatic Metastasis
030220 oncology & carcinogenesis
Thyroidectomy
RNA, Long Noncoding
Proto-Oncogene Proteins B-raf
medicine.medical_specialty
Expert Systems
Biology
Thyroid carcinoma
03 medical and health sciences
Internal medicine
Biomarkers, Tumor
medicine
Humans
Thyroid Neoplasms
Genome wide expression
Neoplasm Staging
Ohio
Gene Expression Profiling
Carcinoma
Biochemistry (medical)
Computational Biology
RNA
Original Articles
Carcinoma, Papillary
Fold change
030104 developmental biology
Amino Acid Substitution
Mutation
Cancer research
Lymph Node Excision
Lymph Nodes
Carcinogenesis
Genome-Wide Association Study
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 101
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....c23db398c37d808432664febb29e6f0a