Supplementary Figure 5 from BRaf and MEK Inhibitors Differentially Regulate Cell Fate and Microenvironment in Human Hepatocellular Carcinoma

Apoptosis induction, cell proliferation and viability after MAPK inhibitor treatment. (A) Apoptosis was determined in HepG2, Hep3B and Huh-7 cells after treatment with 0.2 (light gray bar), 1 (dark gray bar) or 5µM (black bar) inhibitor or DMSO (white bar) at 48h by measuring the subG1 peak using propidium iodide. (B) Cell division was determined by eFluor670 labeling of HepG2, Hep3B and Huh-7 cells after 96h treatment with the different inhibitor concentrations and detected by flow cytometry. (C) The WST-1 metabolic assay was used to detect the viability/proliferation of the three cell lines after treatment with the respective inhibitors for 72h. Each bar represents the mean {plus minus} SDs from three to six replicates.