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Analysis of antibiotic resistance gene expression in Pseudomonas aeruginosa by quantitative real-time-PCR
- Source :
- FEMS Microbiology Letters, Vol. 254, No 2 (2006) pp. 217-225
- Publication Year :
- 2017
-
Abstract
- In Pseudomonas aeruginosa many of the clinically relevant resistance mechanisms result from changes in gene expression as exemplified by the Mex drug efflux pumps, the AmpC beta-lactamase and the carbapenem-specific porin OprD. We used quantitative real-time-PCR to analyze the expression of these genes in susceptible and antibiotic-resistant laboratory and clinical strains. In nalB mutants, which overexpress OprM, we observed a four- to eightfold increase in the expression of mexA, mexB, and oprM genes. MexX and mexY genes were induced eight to 12 times in the presence of 2 mg L(-1) tetracycline. The mexC/oprJ and mexE/oprN gene expression levels were increased 30- to 250-fold and 100- to 760-fold in nfxB and nfxC mutants, respectively. We further found that in defined laboratory strains expression levels of ampC and oprD genes paralleled beta-lactamase activity and OprD protein levels, respectively. Our data support the use of quantitative real-time-PCR chain reaction for the analysis of the antimicrobial resistance gene expression in P. aeruginosa.
- Subjects :
- Tetracycline
Anti-Bacterial Agents/ pharmacology
Mutant
Porins
Drug resistance
Biology
medicine.disease_cause
Microbiology
beta-Lactamases
Reverse Transcriptase Polymerase Chain Reaction/ methods
Bacterial Proteins
Drug Resistance, Bacterial
Gene expression
Genetics
medicine
Humans
Drug Resistance, Bacterial/ genetics
Molecular Biology
Gene
ddc:616
Regulation of gene expression
Reverse Transcriptase Polymerase Chain Reaction
Pseudomonas aeruginosa
Bacterial Proteins/genetics/ metabolism
Gene Expression Regulation, Bacterial
biochemical phenomena, metabolism, and nutrition
Porins/genetics/metabolism
Anti-Bacterial Agents
Beta-Lactamases/genetics/metabolism
Pseudomonas aeruginosa/ drug effects/genetics/metabolism
Efflux
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 03781097
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters, Vol. 254, No 2 (2006) pp. 217-225
- Accession number :
- edsair.doi.dedup.....c2163afc90f63e9230fb591fa40e88c4