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Antenatal Microbial Colonization of Mammalian Gut
- Source :
- Reproductive Sciences, Reproductive sciences (Thousand Oaks, Calif.) 26 (2019): 1045–1053. doi:10.1177/1933719118804411, info:cnr-pdr/source/autori:Elisa Borghi, PhD,1 Valentina Massa, PhD,1 Marco Severgnini, PhD,2 Grazia Fazio, PhD,3 Laura Avagliano, MD,1 Elena Menegola, PhD,4 Gaetano Pietro Bulfamante, MD,1 Giulia Morace, PhD,1 and Francesca Borgo, PhD1/titolo:Antenatal Microbial Colonization of Mammalian Gut/doi:10.1177%2F1933719118804411/rivista:Reproductive sciences (Thousand Oaks, Calif.)/anno:2019/pagina_da:1045/pagina_a:1053/intervallo_pagine:1045–1053/volume:26
- Publication Year :
- 2018
- Publisher :
- SAGE Publications, 2018.
-
Abstract
- The widely accepted dogma of intrauterine sterility and initial colonization of the newborn during birth has been blurred by recent observations of microbial presence in meconium, placenta, and amniotic fluid. Given the importance of a maternal-derived in utero infant seeding, it is crucial to exclude potential environmental or procedural contaminations and to assess fetal colonization before parturition. To this end, we analyzed sterilely collected intestinal tissues, placenta, and amniotic fluid from rodent fetuses and tissues from autoptic human fetuses. Total bacterial DNA was extracted from collected samples and analyzed by Next Generation Sequencing (NGS) techniques using hypervariable 16S ribosomal RNA (rRNA) regions (V3-V4). Colonizing microbes were visualized in situ, using labeled probes targeting 16S ribosomal DNA by fluorescent in situ hybridization. The NGS analysis showed the presence of pioneer microbes in both rat and human intestines as well as in rodent placentas and amniotic fluids. Microbial communities showed fetus- and dam-dependent clustering, confirming the high interindividual variability of commensal microbiota even in the antenatal period. Fluorescent in situ hybridization analysis confirmed the microbes’ presence in the lumen of the developing gut. These findings suggest a possible antenatal colonization of the developing mammalian gut.
- Subjects :
- 0301 basic medicine
Amniotic fluid
Placenta
Embryonic Development
In situ hybridization
Biology
Microbiology
03 medical and health sciences
0302 clinical medicine
Meconium
Pregnancy
RNA, Ribosomal, 16S
medicine
Animals
Humans
Colonization
Ribosomal DNA
reproductive and urinary physiology
16S rRNA gene sequencing
Fetus
030219 obstetrics & reproductive medicine
Microbiota
Obstetrics and Gynecology
mammalian gut
Original Articles
Ribosomal RNA
Amniotic Fluid
Rats
Intestines
030104 developmental biology
medicine.anatomical_structure
Female
Subjects
Details
- Language :
- English
- ISSN :
- 19337205 and 19337191
- Volume :
- 26
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Reproductive Sciences
- Accession number :
- edsair.doi.dedup.....c2100302ea7f6a971691e1bfb4dd7f05