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Analytical and biological methods for probing the blood-brain barrier
- Source :
- Annual review of analytical chemistry (Palo Alto, Calif.). 5
- Publication Year :
- 2012
-
Abstract
- The blood-brain barrier (BBB) is an important interface between the peripheral and central nervous systems. It protects the brain against the infiltration of harmful substances and regulates the permeation of beneficial endogenous substances from the blood into the extracellular fluid of the brain. It can also present a major obstacle in the development of drugs that are targeted for the central nervous system. Several methods have been developed to investigate the transport and metabolism of drugs, peptides, and endogenous compounds at the BBB. In vivo methods include intravenous injection, brain perfusion, positron emission tomography, and microdialysis sampling. Researchers have also developed in vitro cell-culture models that can be employed to investigate transport and metabolism at the BBB without the complication of systemic involvement. All these methods require sensitive and selective analytical methods to monitor the transport and metabolism of the compounds of interest at the BBB.
- Subjects :
- Microdialysis
Central nervous system
Cell Culture Techniques
Endogeny
Pharmacology
Blood–brain barrier
Mass Spectrometry
Article
Analytical Chemistry
In vivo
Extracellular fluid
medicine
Animals
Humans
Chemistry
Brain
Electrophoresis, Capillary
Biological Transport
Equipment Design
Microfluidic Analytical Techniques
medicine.disease
Perfusion
medicine.anatomical_structure
Blood-Brain Barrier
Positron-Emission Tomography
Injections, Intravenous
Infiltration (medical)
Chromatography, Liquid
Subjects
Details
- ISSN :
- 19361335
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Annual review of analytical chemistry (Palo Alto, Calif.)
- Accession number :
- edsair.doi.dedup.....c20bf34c9e57100b67f4388daa851163