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Baseline circulating stem-like cells predict survival in patients with metastatic breast Cancer
- Source :
- BMC Cancer, Vol 19, Iss 1, Pp 1-10 (2019), BMC Cancer
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Background Circulating tumor cells (CTCs) are associated with breast cancer prognosis. Research is limited regarding the role of circulating cancer stem-like cells (cCSCs) considering the treatment response and survival among patients with metastatic breast cancer. Accordingly, we performed this prospective study to clarify the prognostic significance of baseline cCSCs for metastatic breast cancer in terms of first-line chemotherapy. Methods Between April 2014 and January 2016, we prospectively enrolled 48 patients with stage IV breast invasive ductal carcinoma who underwent first-line chemotherapy. We identified and analyzed CTCs and cCSCs by using a protocol based on negative selection and flow cytometry before chemotherapy. CTCs were identified as EpCAM+Hoechst+CD45– cells and cCSCs as CD133+EpCAM+Hoechst+CD45– cells. cCSCs were expressed as a percentage of CTCs. The associations between CTCs, cCSCs, and the clinicopathological variables that were predictive of the treatment response and survival outcome were analyzed using univariate and multivariate analyses. Results We identified CTCs in all the enrolled patients, with a median number of 33.9/mL CTCs. CSCs were isolated in 97.9% of the patients; the median percentage of cCSCs was 14.7%. A high baseline level of cCSCs was correlated with an inferior tumor response rate (54.2% vs. 95.8%, p Conclusions Baseline cCSCs predict the treatment response as well as survival in patients with metastatic breast cancer undergoing first-line chemotherapy. Therefore, the measurement of cCSCs may assist in identifying early cancer treatment response and prognosis.
- Subjects :
- Adult
0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
medicine.medical_treatment
Breast Neoplasms
Cell Count
lcsh:RC254-282
03 medical and health sciences
Breast cancer
0302 clinical medicine
Circulating tumor cell
Cancer stem cell
Surgical oncology
Internal medicine
Genetics
medicine
Humans
Chemotherapy
AC133 Antigen
Prospective Studies
CD133
Prospective cohort study
Aged
Aged, 80 and over
Cancer stem cells
business.industry
Carcinoma, Ductal, Breast
Liquid Biopsy
Circulating tumor cells
Cancer
Middle Aged
Neoplastic Cells, Circulating
Prognosis
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Survival Analysis
Metastatic breast cancer
Treatment Outcome
030104 developmental biology
030220 oncology & carcinogenesis
Neoplastic Stem Cells
Female
business
Research Article
Subjects
Details
- ISSN :
- 14712407
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- BMC Cancer
- Accession number :
- edsair.doi.dedup.....c2033479b0016ca241d9d1ca8cea894e