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Disrupted placental serotonin synthetic pathway and increased placental serotonin
- Source :
- Placenta, Placenta, Elsevier, 2019, 84, pp.74-83. ⟨10.1016/j.placenta.2019.05.012⟩, Placenta, 2019, 84, pp.74-83. ⟨10.1016/j.placenta.2019.05.012⟩, Placenta, 84, 74-83. W B SAUNDERS CO LTD
- Publication Year :
- 2019
-
Abstract
- Objectives: Placental insufficiency contributes to altered maternal-fetal amino acid transfer, and thereby to poor fetal growth. An important placental function is the uptake of tryptophan and its metabolism to serotonin (5-HT) and kynurenine metabolites, which are essential for fetal development. We hypothesised that placental 5-HT content will be increased in pregnancies affected with fetal growth restriction (FGR).Methods: The components of the 5-HT synthetic pathway were determined in chorionic villus samples (CVS) from small-for gestation (SGA) and matched control collected at 10-12 weeks of human pregnancy; and in placentae from third trimester FGR and gestation-matched control pregnancies using the Fluidigm Biomarker array for mRNA expression, the activity of the enzyme TPH and 5-HT concentrations using an ELISA.Results: Gene expression for the rate limiting enzymes, TPH1 and TPH2; 5-HT transporter, SLC6A4; and 5-HT receptors HTR5A, HTR5B, HTR1D and HTR1E were detected in all CVS and third trimester placentae. No significant difference in mRNA was observed in SGA compared with control. Although there was no significant change in TPH1 mRNA, the mRNA of TPH2 and SLC6A4 was significantly decreased in FGR placentae (p Conclusion: This study reports differential expression and activity of the key components of the 5-HT synthetic pathway associated with the pathogenesis of FGR. Further studies are required to elucidate the functional consequences of increased placental 5-HT in FGR pregnancies.
- Subjects :
- Adult
0301 basic medicine
Serotonin
Placenta
Pregnancy Trimester, Third
ENDOMETRIUM
Intrauterine growth restriction
INFANTS
Placental insufficiency
Tryptophan Hydroxylase
Biology
Article
Preeclampsia
Andrology
03 medical and health sciences
0302 clinical medicine
Pregnancy
medicine
PREGNANCIES
Humans
Serotonin Plasma Membrane Transport Proteins
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health
Fetus
Fetal Growth Retardation
030219 obstetrics & reproductive medicine
TPH1
CONSEQUENCES
TPH2
Obstetrics and Gynecology
Placental Insufficiency
medicine.disease
VESICULAR MONOAMINE TRANSPORTER
RELATIVE GENE-EXPRESSION
Up-Regulation
PREECLAMPSIA
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Case-Control Studies
Chorionic villi
Gestation
UPDATE
Female
IMPLANTATION
VILLOUS TREE
Metabolic Networks and Pathways
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 01434004
- Volume :
- 84
- Database :
- OpenAIRE
- Journal :
- Placenta
- Accession number :
- edsair.doi.dedup.....c1fc7fb07888c0aca66a493fd069f8b2