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Dextran sulphate inhibits an association of prions with plasma membrane at the early phase of infection
- Source :
- Neuroscience Research. 171:34-40
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- The defining characteristic of prion diseases is conversion of a cellular prion protein (PrPC) to an abnormal prion protein (PrPSc). The exogenous attachment of PrPSc to the surface of a target cell is critical for infection. However, the initial interaction of PrPSc with the cell surface is poorly characterized. In the current study, we specifically focused on the association of PrPSc with cells during the early phase of infection, using an acute infection model. First, we treated mouse neuroblastoma N2a-58 cells with prion strain 22 L-infected brain homogenates and revealed that PrPSc was associated with membrane fractions within three hours, a short exposure time. These results were also observed in PrPC-deficient hippocampus cell lines. We also demonstrate here that PrPSc from 22 L-infected brain homogenates was associated with lipid rafts during the early phase of infection. Furthermore, we revealed that DS500, a glycosaminoglycan mimetic, inhibited both the attachment of PrPSc to membrane fractions and subsequent prion transmission, suggesting that the early association of prions with cell surface is important for prion infection.
- Subjects :
- 0301 basic medicine
PrPSc Proteins
Prions
animal diseases
Cell
Hippocampus
Endocytosis
Prion Diseases
Glycosaminoglycan
Mice
03 medical and health sciences
0302 clinical medicine
Neuroblastoma
medicine
Animals
Lipid raft
Chemistry
General Neuroscience
Cell Membrane
Dextran Sulfate
General Medicine
medicine.disease
nervous system diseases
Cell biology
030104 developmental biology
Membrane
medicine.anatomical_structure
nervous system
Cell culture
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 01680102
- Volume :
- 171
- Database :
- OpenAIRE
- Journal :
- Neuroscience Research
- Accession number :
- edsair.doi.dedup.....c1f39dd070769904774e969f01c941d3