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Platelet Rich Fibrin (PRF) and Its Related Products: Biomolecular Characterization of the Liquid Fibrinogen
- Source :
- Journal of Clinical Medicine, Volume 9, Issue 4, Journal of Clinical Medicine, Vol 9, Iss 1099, p 1099 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Liquid fibrinogen is an injectable platelet concentrate rich in platelets, leukocytes, and fibrinogen obtained by blood centrifugation. The aim of this study was to analyze the release of different growth factors in the liquid fibrinogen at different times and to assess possible correlations between growth factors and cell counts. The concentration of transforming growth factor beta 1 (TGF-&beta<br />1), platelet-derived growth factor-AB (PDGF-AB), platelet-derived growth factor-BB (PDGF-BB), bone morphogenetic protein 2 (BMP-2), fibroblast growth factor 2 (FGF-2) and vascular endothelial growth factor (VEGF) released by liquid fibrinogen were examined with ELISA at three time points (T0, time of collection<br />T7, 7 days<br />T14, 14 days). The cellular content of the liquid fibrinogen and whole blood was also calculated for each volunteer. A mean accumulation of platelets of almost 1.5-fold in liquid fibrinogen compared to whole blood samples was found. An increase of TGF-&beta<br />1, PDGF-AB, FGF-2, and VEGF levels was detected at T7. At T14, the level of TGF-&beta<br />1 returned to T0 level<br />PDGF-AB amount remained high<br />the levels of FGF-2 and VEGF decreased with respect to T7, but remained higher than the T0 levels<br />PDGF-BB was high at all time points<br />BMP-2 level was low and remained constant at all time points. TGF-&beta<br />1, PDGF-AB, and PDGF-BB showed a correlation with platelet amount, whereas BMP-2, FGF-2, and VEGF showed a mild correlation with platelet amount. Due to the high concentration of platelets, liquid fibrinogen does contain important growth factors for the regeneration of both soft and hard tissue. The centrifugation protocol tested in this study provides a valid solution to stimulate wound healing in oral and periodontal surgery.
- Subjects :
- 0106 biological sciences
medicine.medical_specialty
PRF
growth factors
leukocyte
liquid fibrinogen
platelet concentrate
platelet rich fibrin
platelets
lcsh:Medicine
Fibroblast growth factor
Fibrinogen
01 natural sciences
Article
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
010608 biotechnology
Internal medicine
medicine
Platelet
Centrifugation
Whole blood
business.industry
lcsh:R
030206 dentistry
General Medicine
Platelet-rich fibrin
Vascular endothelial growth factor
Endocrinology
chemistry
business
Wound healing
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20770383
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Medicine
- Accession number :
- edsair.doi.dedup.....c1da593df13152aed3a57f5b4f22cb9b
- Full Text :
- https://doi.org/10.3390/jcm9041099