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NMR spectroscopy of the neuronal tau protein: normal function and implication in Alzheimer's disease
- Source :
- Biochemical Society Transactions, Biochemical Society Transactions, Portland Press, 2010, 38 (4), pp.1006-11. ⟨10.1042/BST0381006⟩, Biochemical Society Transactions, 2010, 38 (4), pp.1006-11. ⟨10.1042/BST0381006⟩
- Publication Year :
- 2010
-
Abstract
- International audience; NMR spectroscopy was used to explore the different aspects of the normal and pathological functions of tau, but proved challenging because the protein contains 441 amino acids and has poor signal dispersion. We have set out to dissect the phosphorylation patterns of tau in order to understand better its role in the aggregation process and microtubule-binding regulation. Our current knowledge on the functional consequences of specific phosphorylations is still limited, mainly because producing and assessing quantitatively phosphorylated tau samples is far from straightforward, even in vitro. We use NMR spectroscopy as a proteomics tool to characterize the phosphorylation patterns of tau, after in vitro phosphorylation by recombinant kinases. The phosphorylated tau can next be use for functional assays or interaction assays with phospho-dependent protein partners, such as the prolyl cis-trans isomerase Pin1.
- Subjects :
- Models, Molecular
Tau protein
MESH: Neurons
tau Proteins
Isomerase
Proteomics
Biochemistry
03 medical and health sciences
Alzheimer Disease
MESH: Nuclear Magnetic Resonance, Biomolecular
Animals
Humans
MESH: Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]
Phosphorylation
MESH: Protein Kinases
Nuclear Magnetic Resonance, Biomolecular
030304 developmental biology
chemistry.chemical_classification
Neurons
0303 health sciences
MESH: Humans
MESH: Phosphorylation
biology
Kinase
030302 biochemistry & molecular biology
Nuclear magnetic resonance spectroscopy
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM]
MESH: tau Proteins
Amino acid
chemistry
PIN1
biology.protein
Protein Kinases
MESH: Alzheimer Disease
MESH: Models, Molecular
Subjects
Details
- ISSN :
- 14708752 and 03005127
- Volume :
- 38
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Biochemical Society transactions
- Accession number :
- edsair.doi.dedup.....c1da2ed025b8d8b7e3d66c14d9d171ad