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Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep
- Source :
- Reproduction (Cambridge, England). 150(2)
- Publication Year :
- 2014
-
Abstract
- The aim of the present study was to determine the direct cause of the mutation-induced, increased ovulation rate in Booroola Merino (BB) sheep. Granulosa cells were removed from antral follicles before ovulation and post-ovulation from BB (n=5) and WT (n=12) Merino ewes. Direct immunofluorescence measurement of mature cell surface receptors using flow cytometry demonstrated a significant up-regulation of FSH receptor (FSHR), transforming growth factor beta type 1, bone morphogenetic protein receptor (BMPR1B), and LH receptor (LHR) in BB sheep. The increased density of FSHR and LHR provide novel evidence of a mechanism for increasing the number of follicles that are recruited during dominant follicle selection. The compounding increase in receptors with increasing follicle size maintained the multiple follicles and reduced the apoptosis, which contributed to a high ovulation rate in BB sheep. In addition, we report a mutation-independent mechanism of down-regulation to reduce receptor density of the leading dominant follicle in sheep. The suppression of receptor density coincides with the cessation of mitogenic growth and steroidogenic differentiation as part of the luteinization of the follicle. The BB mutation-induced attenuation of BMPR1B signaling led to an increased density of the FSHR and LHR and a concurrent reduction in apoptosis to increase the ovulation rate. The role of BMPs in receptor modulation is implicated in the development of multiple ovulations.
- Subjects :
- Ovulation
Embryology
medicine.medical_specialty
media_common.quotation_subject
Apoptosis
Follicle
Endocrinology
Ovarian Follicle
Pregnancy
Internal medicine
medicine
Animals
Ovarian follicle
Receptor
Bone Morphogenetic Protein Receptors, Type I
Sheep, Domestic
media_common
Granulosa Cells
biology
luteinizing hormone/choriogonadotropin receptor
Obstetrics and Gynecology
Cell Biology
Transforming growth factor beta
Receptors, LH
Antral follicle
Flow Cytometry
medicine.anatomical_structure
Reproductive Medicine
Mutation
biology.protein
Receptors, FSH
Female
Steroids
Follicle-stimulating hormone receptor
Subjects
Details
- ISSN :
- 17417899
- Volume :
- 150
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Reproduction (Cambridge, England)
- Accession number :
- edsair.doi.dedup.....c1d7a9a445c0f0a812965815575cf9ea