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Doxycycline Changes the Transcriptome Profile of mIMCD3 Renal Epithelial Cells

Authors :
Hyun Jun Jung
Richard Coleman
Owen M. Woodward
Paul A. Welling
Source :
Frontiers in Physiology, Vol 12 (2021), Frontiers in Physiology
Publication Year :
2021
Publisher :
Frontiers Media SA, 2021.

Abstract

Tetracycline-inducible gene expression systems have been used successfully to study gene function in vivo and in vitro renal epithelial models but the effects of the common inducing agent, doxycycline (DOX), on gene expression are not well appreciated. Here, we evaluated the DOX effects on the transcriptome of a widely used renal epithelial cell model, mIMCD3 cells, to establish a reference. Cells were grown on permeable filter supports in the absence and presence of DOX (3 or 6 days), and genome-wide transcriptome profiles were assessed using RNA-Seq. We found DOX significantly altered the transcriptome profile, changing the abundance of 1,549 transcripts at 3 days and 2,643 transcripts at 6 days. Within 3 days of treatment, DOX significantly decreased the expression of multiple signaling pathways (ERK, cAMP, and Notch) that are associated with cell proliferation and differentiation. Genes associated with cell cycle progression were subsequently downregulated in cells treated with DOX for 6 days, as were genes involved in cellular immune response processes and several cytokines and chemokines, correlating with a remarkable repression of genes encoding cell proliferation markers. The results provide new insight into responses of renal epithelial cells to DOX and a establish a resource for DOX-mediated gene expression systems.

Details

Language :
English
ISSN :
1664042X
Volume :
12
Database :
OpenAIRE
Journal :
Frontiers in Physiology
Accession number :
edsair.doi.dedup.....c1b9d97bd1f7a54df66c3d01e42c0832
Full Text :
https://doi.org/10.3389/fphys.2021.771691