Back to Search
Start Over
HCV796: A selective nonstructural protein 5B polymerase inhibitor with potent anti-hepatitis C virus activity in vitro, in mice with chimeric human livers, and in humans infected with hepatitis C virus
- Source :
- Hepatology (Baltimore, Md.). 49(3)
- Publication Year :
- 2008
-
Abstract
- Anti-hepatitis C virus (HCV) drug development has been challenged by a lack of experience with inhibitors inclusive of in vitro, animal model, and clinical study. This manuscript outlines activity and correlation across such a spectrum of models and into clinical trials with a novel selective nonstructural protein 5B (NS5B) polymerase inhibitor, HCV796. Enzyme assays yielded median inhibitory concentration (IC50) values of 0.01 to 0.14 μM for genotype 1, with half maximal effective concentration (EC50s) of 5 nM and 9 nM against genotype 1a and 1b replicons. In the chimeric mouse model, a 2.02 ± 0.55 log reduction in HCV titer was seen with monotherapy, whereas a suboptimal dose of 30 mg/kg three times per day in combination with interferon demonstrated a 2.44 log reduction (P = 0.001 versus interferon alone) Clinical outcomes in combination with pegylated interferon and ribavirin have revealed additive efficacy in treatment naive patients. Abnormal liver function test results were observed in 8% of HCV-796 patients treated for over 8 weeks, resulting in suspension of further trial activity. Conclusion: The RNA-dependent RNA polymerase inhibitor HCV796 demonstrated potent anti-HCV activity consistently through enzyme inhibition assays, subgenomic replicon, and chimeric mouse studies. Strong correlations of outcomes in the mouse model were seen with subsequent clinical trials, including a plateau in dose-related antiviral activity and additive impact from combination therapy with interferon. These outcomes demonstrate the utility of the range of in vitro and in vivo models now available for anti-HCV drug development and support the potential utility of polymerase inhibitors in future combination therapies for HCV treatment. (HEPATOLOGY 2009.)
- Subjects :
- Hepatitis C virus
Hepacivirus
Mice, SCID
Biology
Interferon alpha-2
Viral Nonstructural Proteins
medicine.disease_cause
Antiviral Agents
Virus
Polyethylene Glycols
chemistry.chemical_compound
Mice
Double-Blind Method
In vivo
Pegylated interferon
Interferon
Ribavirin
medicine
Animals
Humans
Enzyme Inhibitors
NS5B
Benzofurans
Sulfonamides
Hepatology
Dose-Response Relationship, Drug
Interferon-alpha
Hepatitis C
medicine.disease
Virology
Recombinant Proteins
Disease Models, Animal
chemistry
Liver
Hepatocytes
Drug Therapy, Combination
Replicon
medicine.drug
Subjects
Details
- ISSN :
- 15273350
- Volume :
- 49
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Hepatology (Baltimore, Md.)
- Accession number :
- edsair.doi.dedup.....c1a8e07d6dbbb1c921571747e2544858