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Proteins encoded by the conservon of Streptomyces coelicolor A3(2) comprise a membrane-associated heterocomplex that resembles eukaryotic G protein-coupled regulatory system
- Source :
- Molecular Microbiology. 62:1534-1546
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- Summary Streptomyces coelicolor A3(2) retains unique conserved operons termed conservons. Here, one of the conservons (cvn9), which encodes five proteins (A9-E9), was characterized. Mutants for cvnA9 and cvnA10 conditionally overproduced actinorhodin and performed precocious aerial growth, while a cvnE9 mutant showed the parental phenotype. Transcription of bldG, adpA and bldN was upregulated in the cvnA9 mutant. A9-D9 were detected in the insoluble fraction of cell-free extract of S. coelicolor by Western analysis. Biochemical analyses revealed that A9 has ATP-hydrolysing and adenine nucleotide-binding activities; D9 has GTP-hydrolysing and guanine nucleotide-binding activities; and E9 shows a typical spectrum similar to cytochrome P450. The comprehensive interaction assays demonstrated the occurrence of specific interactions between A9 and B9, A9 and C9, B9 and B9, B9 and D9, and C9 and D9. A9 associated with and dissociated from B9 (and C9) when ATP and ATP-γ-S were supplied in the reaction respectively. Similarly, D9 associated with and dissociated from B9 (and C9) when GTP and GTP-γ-S were supplied respectively. A9 and B9 were also shown for the occurrence as homocomplexes. Probably, Cvn9 proteins comprise a membrane-associated heterocomplex resembling the eukaryotic G-protein-coupled receptor system, which may serve as a signal transducer that connects to the bld cascade.
- Subjects :
- GTP'
G protein
Operon
Blotting, Western
Mutant
Streptomyces coelicolor
Models, Biological
Microbiology
Actinorhodin
chemistry.chemical_compound
Adenosine Triphosphate
Bacterial Proteins
GTP-Binding Proteins
Transcription (biology)
Two-Hybrid System Techniques
Molecular Biology
biology
biology.organism_classification
Phenotype
Biochemistry
chemistry
Multiprotein Complexes
Mutation
Electrophoresis, Polyacrylamide Gel
Guanosine Triphosphate
Actinomycetales
Protein Binding
Subjects
Details
- ISSN :
- 13652958 and 0950382X
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Molecular Microbiology
- Accession number :
- edsair.doi.dedup.....c1a6787be36e109b01b26552c3f1bbd3