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Molecular Requirements for MHC Class II α-Chain Engagement and Allelic Discrimination by the Bacterial Superantigen Streptococcal Pyrogenic Exotoxin C
- Source :
- The Journal of Immunology. 181:3384-3392
- Publication Year :
- 2008
- Publisher :
- The American Association of Immunologists, 2008.
-
Abstract
- Superantigens (SAgs) are microbial toxins that bind to both TCR β-chain variable domains (Vβs) and MHC class II molecules, resulting in the activation of T cells in a Vβ-specific manner. It is now well established that different isoforms of MHC II molecules can play a significant role in the immune response to bacterial SAgs. In this work, using directed mutational studies in conjunction with functional analyses, we provide a complete functional map of the low-affinity MHC II α-chain binding interface of the SAg streptococcal pyrogenic exotoxin C (SpeC) and identify a functional epitope in the β-barrel domain that is required for the activation of T cells. Using cell lines that exclusively express individual MHC II isoforms, our studies provide a molecular basis for the selectivity of SpeC-MHC II recognition, and provide one mechanism by how SAgs are capable of distinguishing between different MHC II alleles.
- Subjects :
- Genetics
MHC class II
CD74
T-Lymphocytes
Immunology
T-cell receptor
Histocompatibility Antigens Class II
Exotoxins
chemical and pharmacologic phenomena
Biology
MHC restriction
Lymphocyte Activation
Epitope
Cell Line
Epitopes
Jurkat Cells
Bacterial Proteins
MHC class I
biology.protein
Superantigen
Humans
Immunology and Allergy
Protein Interaction Domains and Motifs
Epitope Mapping
Alpha chain
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 181
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi.dedup.....c1a10b14ea1b41668f1b5bd49c980e0a