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A global phylogenetic analysis of Japanese tonsil-derived Epstein-Barr virus strains using viral whole-genome cloning and long-read sequencing
- Source :
- The Journal of general virology. 102(3)
- Publication Year :
- 2021
-
Abstract
- Epstein–Barr virus (EBV) establishes lifelong latent infection in the majority of healthy individuals, while it is a causative agent for various diseases, including some malignancies. Recent high-throughput sequencing results indicate that there are substantial levels of viral genome heterogeneity among different EBV strains. However, the extent of EBV strain variation among asymptomatically infected individuals remains elusive. Here, we present a streamlined experimental strategy to clone and sequence EBV genomes derived from human tonsillar tissues, which are the reservoirs of asymptomatic EBV infection. Complete EBV genome sequences, including those of repetitive regions, were determined for seven tonsil-derived EBV strains. Phylogenetic analyses based on the whole viral genome sequences of worldwide non-tumour-derived EBV strains revealed that Asian EBV strains could be divided into several distinct subgroups. EBV strains derived from nasopharyngeal carcinoma-endemic areas constitute different subgroups from a subgroup of EBV strains from non-endemic areas, including Japan. The results could be consistent with biased regional distribution of EBV-associated diseases depending on the different EBV strains colonizing different regions in Asian countries.
- Subjects :
- 0301 basic medicine
Chromosomes, Artificial, Bacterial
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Genes, Viral
Palatine Tonsil
Clone (cell biology)
Genome, Viral
Biology
medicine.disease_cause
Genome
Virus
Cell Line
03 medical and health sciences
Viral Proteins
0302 clinical medicine
Japan
hemic and lymphatic diseases
Virology
medicine
Humans
Lymphocytes
Cloning, Molecular
Asymptomatic Infections
Phylogeny
Repetitive Sequences, Nucleic Acid
Cloning
Bacterial artificial chromosome
Phylogenetic tree
Whole Genome Sequencing
Genetic Variation
High-Throughput Nucleotide Sequencing
Sequence Analysis, DNA
Epstein–Barr virus
Virus Latency
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Tonsil
DNA, Viral
Subjects
Details
- ISSN :
- 14652099
- Volume :
- 102
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Journal of general virology
- Accession number :
- edsair.doi.dedup.....c18be5e53f6ec9e2be469c2c56bd1752