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Circulating cell‐free DNA release in vitro: kinetics, size profiling, and cancer‐related gene methylation
- Source :
- Journal of Cellular Physiology. 234:14079-14089
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Circulating cell-free DNA (ccfDNA) is a biological entity of great interest due to its potential as liquid biopsy biomaterial carrying clinically valuable information. To better understand its nature, we studied ccfDNA in vitro in two human cancer cell lines MCF-7 and HeLa. Normalized indexes of ccfDNA per cell population decreased over time of culture but were significantly elevated after exposure to IC50 doses of the demethylating/apoptotic agent 5-azacytidine (5-AZA-CR). Fragment-size profiling was indicative of active release, whereas exposure to 5-AZA-CR induced the release of additional shorter fragments, indicative of apoptosis. Finally, the methylation profile of a panel of cancer-specific genes as assessed by quantitative methylation analysis in ccfDNA was identical to the corresponding genomic DNA and followed accurately changes caused by 5-AZA-CR. Overall, our in vitro findings support that ccfDNA can be a reliable biosource of clinically relevant information that can be further studied in these cell culture models.
- Subjects :
- 0301 basic medicine
Physiology
Clinical Biochemistry
Population
Cell
HeLa
03 medical and health sciences
0302 clinical medicine
Neoplasms
Biomarkers, Tumor
medicine
Humans
Liquid biopsy
education
education.field_of_study
biology
Chemistry
Cell Biology
Methylation
DNA Methylation
biology.organism_classification
Molecular biology
Circulating Cell-Free DNA
Kinetics
030104 developmental biology
medicine.anatomical_structure
Cell culture
Apoptosis
030220 oncology & carcinogenesis
Azacitidine
MCF-7 Cells
Cell-Free Nucleic Acids
HeLa Cells
Subjects
Details
- ISSN :
- 10974652 and 00219541
- Volume :
- 234
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Physiology
- Accession number :
- edsair.doi.dedup.....c18b68a585450bc620373c2076813106