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Regulatory roles of claudin-1 in cell adhesion and microvilli formation

Authors :
Tomoyuki Aoyama
Taishi Akimoto
Daisuke Kyuno
Taro Murakami
Hiroshi Kitajima
Hiromu Suzuki
Kumi Takasawa
Makoto Osanai
Kazufumi Magara
Akira Takasawa
Yusuke Ono
Source :
Biochemical and Biophysical Research Communications. 565:36-42
Publication Year :
2021
Publisher :
Elsevier BV, 2021.

Abstract

Aberrant expression of tight junction proteins has recently been focused on in the cancer research field. We previously showed that claudin-1 is aberrantly expressed from an early stage of uterine cervical adenocarcinoma and contributes to malignant potentials. To elucidate the molecular mechanisms underlying tumor-promoting roles of claudin-1, we established and analyzed claudin-1 knockout cells. Knockout of claudin-1 suppressed conventional tight junctional functions, barrier and fence functions, and expression of cell adhesion-associated proteins including E-cadherin. Comparative proteome analysis revealed that expression of claudin-1 affected expression of a wide range of proteins, especially proteins that are associated with cell adhesion and actin cytoskeleton remodeling. Interactome analysis of the identified proteins revealed that E-cadherin and focal adhesion kinase play central roles in the claudin-1-dependently affected protein network. Moreover, knockout of claudin-1 significantly suppressed microvilli formation and activity of Ezrin/Radixin/Moesin. Taken together, the results indicate that expression of claudin-1 affects not only conventional tight junction function but also expression and activity of a wide range of proteins, especially proteins that are associated with cell adhesion and actin cytoskeleton remodeling, to contribute to malignant potentials and microvilli formation in cervical adenocarcinoma cells.

Details

ISSN :
0006291X
Volume :
565
Database :
OpenAIRE
Journal :
Biochemical and Biophysical Research Communications
Accession number :
edsair.doi.dedup.....c17f777974d82dee4c42e180f8b6add7
Full Text :
https://doi.org/10.1016/j.bbrc.2021.05.070