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GC-MS analysis of Myrtus communis extract and its antibacterial activity against Gram-positive bacteria
- Source :
- BMC Complementary Medicine and Therapies, BMC Complementary Medicine and Therapies, Vol 20, Iss 1, Pp 1-9 (2020)
- Publication Year :
- 2019
-
Abstract
- Background Myrtus communis is a typical plant of Mediterranean area. The different parts of this plant such as berries, branches, and leaves have been used worldwide as a traditional/folk medicine for the treatment of various ailments and diseases. Methods Ethanolic leaf extract of the plant was prepared by Soxhlet extraction method. Zone of inhibition, minimum inhibitory concentration and minimal bactericidal concentration were determined by well diffusion method and microplate alamar blue assay. GC-MS analysis was carried out to identify the compounds present in the extract. Microscopy and ImageJ software were used respectively for morphology and cell-length measurements. GraphPad Prism was used for statistical analysis. Results The ethanolic extract showed strong inhibitory effect against Gram-positive and acid-fast bacteria with significant inhibition-zone size (9–25 mm), MIC (4.87–78 μg/ml), as well as MBC (0.3–20 mg/ml). However, no effect was observed on the growth of Gram-negative bacteria. The growth inhibition was found to be associated with the damage of cell wall as the extract-treated cells were sensitive to cell wall-targeting antibiotics and displayed the cell wall damage-depicting morphological defects. GC-MS analysis confirmed the presence of novel compounds in addition to the most representative compounds of the essential oils/extracts of M. communis of other country origins. Conclusion These results demonstrate that M. communis leaf extract could be the source of compounds to be used for the treatment of Gram-positive bacterial infections. This is the first report, which provides insights into the mechanism of action of the extract in inhibiting the growth of Gram-positive bacteria.
- Subjects :
- 0301 basic medicine
Gram-negative bacteria
Gram-positive bacteria
Saudi Arabia
Microbial Sensitivity Tests
Gas Chromatography-Mass Spectrometry
03 medical and health sciences
Minimum inhibitory concentration
Myrtus communis
0302 clinical medicine
Agar diffusion test
Minimum bactericidal concentration
biology
Traditional medicine
Chemistry
Plant Extracts
Cell wall
lcsh:Other systems of medicine
lcsh:RZ201-999
biology.organism_classification
Myrtus
Anti-Bacterial Agents
Plant Leaves
Plant extract
030104 developmental biology
Complementary and alternative medicine
030220 oncology & carcinogenesis
GC-MS
Antibacterial activity
Bacteria
Research Article
Subjects
Details
- ISSN :
- 26627671
- Volume :
- 20
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC complementary medicine and therapies
- Accession number :
- edsair.doi.dedup.....c17d99252d0839ff6c5b6a71d35884e0