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Multi-lamellar organization of fully deuterated lipid extracts of yeast membranes

Authors :
Michael Haertlein
Yuri Gerelli
Valerie Laux
Alexis de Ghellinck
Juliette Jouhet
Giovanna Fragneto
Institut Laue-Langevin (ILL)
ILL
Département de physique-Université Libre de Bruxelles
Département de physique
Laboratoire de physiologie cellulaire végétale (LPCV)
Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)
European Project: 283883,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2011-1,NMI3-II(2012)
European Project: RII3-CT- 2003505925,Deuteration consortium
European Project: EP/C015452/1,UK EPSRC-funded activity for the initial creation of the Deuteration Laboratory.
Département de Physique [Bruxelles] (ULB)
Faculté des Sciences [Bruxelles] (ULB)
Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB)
European Commission [283883]
EU [RII3-CT-2003505925]
UK EPSRC [EP/C015452/1]
Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)
Direction de Recherche Fondamentale (CEA) (DRF (CEA))
Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA))
Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
Source :
Acta Crystallographica Section D: Biological Crystallography, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩, Acta crystallographica Section D : Structural biology [1993-...], Acta crystallographica Section D : Structural biology [1993-..], 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩
Publication Year :
2014
Publisher :
HAL CCSD, 2014.

Abstract

Neutron scattering studies on mimetic biomembranes are currently limited by the low availability of deuterated unsaturated lipid species. In the present work, results from the first neutron diffraction experiments on fully deuterated lipid extracts from the yeastPichia pastorisare presented. The structural features of these fully deuterated lipid stacks are compared with those of their hydrogenous analogues and with other similar synthetic systems. The influence of temperature and humidity on the samples has been investigated by means of small momentum-transfer neutron diffraction. All of the lipid extracts investigated self-assemble into multi-lamellar stacks having different structural periodicities; the stacking distances are affected by temperature and humidity without altering the basic underlying arrangement. At high relative humidity the deuterated and hydrogenous samples are similar in their multi-lamellar arrangement, being characterized by two main periodicities of ∼75 and ∼110 Å reflecting the presence of a large number of polar phospholipid molecules. Larger differences are found at lower relative humidity, where hydrogenous lipids are characterized by a larger single lamellar structure than that observed in the deuterated samples. In both cases the heterogeneity in composition is reflected in a wide structural complexity. The different behaviour upon dehydration can be related to compositional differences in the molecular composition of the two samples, which is attributed to metabolic effects related to the use of perdeuterated growth media.

Details

Language :
English
ISSN :
09074449 and 20597983
Database :
OpenAIRE
Journal :
Acta Crystallographica Section D: Biological Crystallography, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩, Acta crystallographica Section D : Structural biology [1993-...], Acta crystallographica Section D : Structural biology [1993-..], 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩
Accession number :
edsair.doi.dedup.....c168dac8ba48a80c14e4847817e1f2c3
Full Text :
https://doi.org/10.1107/S1399004714022913⟩