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Multi-lamellar organization of fully deuterated lipid extracts of yeast membranes
- Source :
- Acta Crystallographica Section D: Biological Crystallography, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩, Acta crystallographica Section D : Structural biology [1993-...], Acta crystallographica Section D : Structural biology [1993-..], 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Neutron scattering studies on mimetic biomembranes are currently limited by the low availability of deuterated unsaturated lipid species. In the present work, results from the first neutron diffraction experiments on fully deuterated lipid extracts from the yeastPichia pastorisare presented. The structural features of these fully deuterated lipid stacks are compared with those of their hydrogenous analogues and with other similar synthetic systems. The influence of temperature and humidity on the samples has been investigated by means of small momentum-transfer neutron diffraction. All of the lipid extracts investigated self-assemble into multi-lamellar stacks having different structural periodicities; the stacking distances are affected by temperature and humidity without altering the basic underlying arrangement. At high relative humidity the deuterated and hydrogenous samples are similar in their multi-lamellar arrangement, being characterized by two main periodicities of ∼75 and ∼110 Å reflecting the presence of a large number of polar phospholipid molecules. Larger differences are found at lower relative humidity, where hydrogenous lipids are characterized by a larger single lamellar structure than that observed in the deuterated samples. In both cases the heterogeneity in composition is reflected in a wide structural complexity. The different behaviour upon dehydration can be related to compositional differences in the molecular composition of the two samples, which is attributed to metabolic effects related to the use of perdeuterated growth media.
- Subjects :
- Chemistry
Lipid Bilayers
Neutron diffraction
Phospholipid
Membrane
perdeuterated growth medium
Humidity
General Medicine
Neutron scattering
yeast
Deuterium
Lipids
Pichia
Fully deuterated lipids extracts
chemistry.chemical_compound
Crystallography
neutron diffraction
Pichia pastoris
Structural Biology
Molecule
Relative humidity
Lamellar structure
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Subjects
Details
- Language :
- English
- ISSN :
- 09074449 and 20597983
- Database :
- OpenAIRE
- Journal :
- Acta Crystallographica Section D: Biological Crystallography, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76, Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩, Acta crystallographica Section D : Structural biology [1993-...], Acta crystallographica Section D : Structural biology [1993-..], 2014, 70 (Pt 12), pp.3167-76. ⟨10.1107/S1399004714022913⟩
- Accession number :
- edsair.doi.dedup.....c168dac8ba48a80c14e4847817e1f2c3
- Full Text :
- https://doi.org/10.1107/S1399004714022913⟩