Back to Search
Start Over
Building a DNA barcode library of Alaska’s non-marine arthropods
- Source :
- Genome. 60:248-259
- Publication Year :
- 2017
- Publisher :
- Canadian Science Publishing, 2017.
-
Abstract
- Climate change may result in ecological futures with novel species assemblages, trophic mismatch, and mass extinction. Alaska has a limited taxonomic workforce to address these changes. We are building a DNA barcode library to facilitate a metabarcoding approach to monitoring non-marine arthropods. Working with the Canadian Centre for DNA Barcoding, we obtained DNA barcodes from recently collected and authoritatively identified specimens in the University of Alaska Museum (UAM) Insect Collection and the Kenai National Wildlife Refuge collection. We submitted tissues from 4776 specimens, of which 81% yielded DNA barcodes representing 1662 species and 1788 Barcode Index Numbers (BINs), of primarily terrestrial, large-bodied arthropods. This represents 84% of the species available for DNA barcoding in the UAM Insect Collection. There are now 4020 Alaskan arthropod species represented by DNA barcodes, after including all records in Barcode of Life Data Systems (BOLD) of species that occur in Alaska — i.e., 48.5% of the 8277 Alaskan, non-marine-arthropod, named species have associated DNA barcodes. An assessment of the identification power of the library in its current state yielded fewer species-level identifications than expected, but the results were not discouraging. We believe we are the first to deliberately begin development of a DNA barcode library of the entire arthropod fauna for a North American state or province. Although far from complete, this library will become increasingly valuable as more species are added and costs to obtain DNA sequences fall.
- Subjects :
- 0106 biological sciences
Canada
Insecta
010607 zoology
Biodiversity
Biology
Barcode
010603 evolutionary biology
01 natural sciences
DNA barcoding
law.invention
Species Specificity
law
Genetics
Animals
DNA Barcoding, Taxonomic
Arthropods
Molecular Biology
Phylogeny
Gene Library
Trophic level
Ecology
Geography
Models, Genetic
Temperature
Genetic Variation
DNA
Sequence Analysis, DNA
General Medicine
biology.organism_classification
Dna barcodes
Wildlife refuge
Arthropod
Alaska
Biotechnology
Subjects
Details
- ISSN :
- 14803321 and 08312796
- Volume :
- 60
- Database :
- OpenAIRE
- Journal :
- Genome
- Accession number :
- edsair.doi.dedup.....c1665003995be3cd142daa82687f0099