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Design of a microfluidic device for the phase-contrast tomography of flowing cells
- Source :
- Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V.
- Publication Year :
- 2021
- Publisher :
- SPIE, 2021.
-
Abstract
- The detection of CTCs in a blood sample is a challenging task due to their rarity and variety. We develop a new label-free and all-optical approach at the lab-on-chip scale for the detection of CTCs based on morphological biomarkers. In particular, we design a microfluidic device to be combined with a phase-contrast tomography system to carry out quantitative measurements of the three-dimensional structure of each single cell in a blood sample. In such device, two aspects are conjugated: on the one hand, the cells need to perform at least one complete rotation within the field of view of the imaging apparatus; on the other hand, the highest possible throughput has to be achieved, yet without deforming the cells significantly, which would impede their tomographic reconstruction. In this contribution, the finite-element-simulation-based preliminary design of a microfluidic device that would allow the achievement of the aforementioned objectives for cells with different shape and deformability is presented.
Details
- Database :
- OpenAIRE
- Journal :
- Optical Methods for Inspection, Characterization, and Imaging of Biomaterials V
- Accession number :
- edsair.doi.dedup.....c157f8de79b2ec73e4771eaf27f4ba76