Back to Search
Start Over
Down-regulation of pro-necroptotic molecules blunts necroptosis during myogenesis
- Source :
- Biochemical and Biophysical Research Communications. 557:33-39
- Publication Year :
- 2021
- Publisher :
- Elsevier BV, 2021.
-
Abstract
- Cell death and differentiation are closely related at the molecular level. Differentiation of skeletal muscle cells attenuates susceptibility to apoptosis. Necroptosis has recently been recognized as a form of regulated cell death but its role in myogenesis has not been studied. This study aimed to compare the sensitivity to TNF-induced necroptosis in skeletal muscle at the undifferentiated (myoblasts) and differentiated (myotubes) stages. Surprisingly, our results showed that TNF-induced necroptosis was blunted during myoblast differentiation. Moreover, our data revealed that the key molecules involved in necroptosis, including receptor-interacting serine/threonine protein kinase 1 (RIPK1), RIPK3, and mixed lineage kinase domain-like protein (MLKL), were significantly down-regulated during myogenic differentiation, resulting in suppression of necroptosis signal transduction in differentiated myotubes. In addition, RIPK1, RIPK3, and MLKL expression levels were significantly lower in the skeletal muscle of adult mice than in newborn mice, suggesting that the susceptibility to necroptosis might be attenuated in differentiated muscle tissue. In conclusion, this study revealed that expression of key molecules involved in necroptosis is down-regulated during muscle differentiation, which results in the differentiation of muscles becoming insensitive to necroptotic cell death.
- Subjects :
- Male
0301 basic medicine
Programmed cell death
Necroptosis
Biophysics
Down-Regulation
Apoptosis
Biology
Muscle Development
Biochemistry
Mice
03 medical and health sciences
RIPK1
0302 clinical medicine
medicine
Animals
Myocyte
Phosphorylation
Muscle, Skeletal
Molecular Biology
Cells, Cultured
Myogenesis
Skeletal muscle
Cell Differentiation
Cell Biology
Cell biology
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Receptor-Interacting Protein Serine-Threonine Kinases
030220 oncology & carcinogenesis
Signal transduction
Protein Kinases
C2C12
Signal Transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 557
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....c156423cfa10a91278b334c4291724d9