Back to Search
Start Over
CD44 and ALDH1 immunoexpression as prognostic indicators of invasion and metastasis in oral squamous cell carcinoma
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2018
- Publisher :
- Wiley, 2018.
-
Abstract
- Background Tumour metastasis has been associated with cancer stem cells, a small population with stem-like cells properties, higher rate of migration and metastatic potential compared to cells from the tumour bulk. Our aim was to evaluate the immunoexpression of the putative cancer stem cell biomarkers ALDH1 and CD44 in primary tumour and corresponding metastatic lymph nodes. Methods Tumour tissue specimens (n = 50) and corresponding metastatic lymph nodes (n = 25) were surgically obtained from 50 patients with oral squamous cell carcinoma and submitted to immunohistochemistry. CD44 and ALDH1 were semi-quantitatively scored according to the proportion and intensity of positive cells within the invasive front and metastatic lymph nodes as a whole. A combined score was obtained by multiplying both parameters and later dichotomized into a final score classified as low (≤2) or high (>2) immunoexpression. Results ALDH1 immunoexpression and CD44 immunoexpression were detected in both tumour sites, although the means of ALDH1 (P = .0985) and CD44 (P = .4220) cells were higher in metastasis compared to primary tumours. ALDH1high was positively associated (P = .0184) with angiolymphatic invasion, while CD44high was positively associated (P = .0181) with metastasis (N+). At multivariate analysis, CD44 significantly increased the odds of lymph node metastasis, regardless of T stage (OR = 8.24; 1.64-65.64, P = .0088). Conclusions CD44 immunoexpression was a significant predictor of lymph node metastasis, while ALDH1high immunostaining was associated with angiolymphatic invasion. Altogether, it suggests that immunoexpression of CD44 and ALDH1 links the cancer stem cell phenotype with oral squamous cell carcinoma invasion and metastasis.
- Subjects :
- Male
0301 basic medicine
Cancer Research
Pathology
medicine.medical_specialty
Population
Aldehyde Dehydrogenase 1 Family
Pathology and Forensic Medicine
Metastasis
03 medical and health sciences
0302 clinical medicine
CARCINOMA DE CÉLULAS ESCAMOSAS
Cancer stem cell
Biomarkers, Tumor
Humans
Medicine
Neoplasm Invasiveness
education
Mouth neoplasm
education.field_of_study
biology
business.industry
CD44
Retinal Dehydrogenase
Middle Aged
Prognosis
medicine.disease
Immunohistochemistry
Isoenzymes
Hyaluronan Receptors
030104 developmental biology
Otorhinolaryngology
Lymphatic Metastasis
030220 oncology & carcinogenesis
Carcinoma, Squamous Cell
Neoplastic Stem Cells
biology.protein
Periodontics
T-stage
Female
Mouth Neoplasms
Lymph
Oral Surgery
business
Subjects
Details
- ISSN :
- 09042512
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Journal of Oral Pathology & Medicine
- Accession number :
- edsair.doi.dedup.....c12fceb6fa4859ae7a65a3b9d4ea1bf8