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Expression patterns and immunolocalisation of IGF-I and IGF-II in male and female gonads of the Neotropical characid fish Astyanax fasciatus
- Source :
- Fish Physiology and Biochemistry. 45:167-176
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- The insulin-like growth factor (IGF) system plays important roles in fish reproduction, but the expression pattern and cellular location of IGF-I and IGF-II during gonadal maturation are uncertain. The present study reports a stage-specific assessment of gonadal expression levels and immunolocalisation of IGF-I and IGF-II in Astyanax fasciatus, a characid fish from South America. Adult fish in different maturity stages were caught in the Furnas Reservoir, Grande River, Brazil. Gonad samples were processed for histology, immunohistochemistry, and ELISA for IGF-I and IGF-II. Ovarian levels of IGF-I were low during ripening and ripe stages, higher in totally spent, and then decreased in resting. Levels of IGF-II increased during ovarian maturation, reaching significantly higher values at stage totally spent. In males, IGF-I levels followed gonadal maturation, with higher values in ripening and ripe stages, whereas IGF-II levels showed higher values in stage ripening and partially spent. A positive correlation was found between IGF-I and gonadosomatic index (GSI) for males (r = 0.59), while females showed a negative correlation (r = - 0.43), but IGF-II showed no correlation to GSI. IGF-I was expressed mainly in oogonia nests whereas IGF-II stained the follicular cells in the perinucleolar follicles, cortical vesicles in the previtellogenic follicles, and oogonia nests. In males, IGF-I was evident in spermatogonia and spermatocytes while IGF-II stained Sertoli cells surrounding spermatids cysts and spermatogonia in late stages. Together, these findings support a hypothesis that the balance between IGF-I and IGF-II levels is important in the regulation of gonad maturation in Astyanax fasciatus.
- Subjects :
- Male
endocrine system
Gonad
Physiology
medicine.medical_treatment
Ovary
Fish reproduction
Aquatic Science
Biology
Biochemistry
Andrology
03 medical and health sciences
Insulin-like growth factor
Insulin-Like Growth Factor II
Testis
Follicular phase
medicine
Animals
Genes, Developmental
Insulin-Like Growth Factor I
030304 developmental biology
0303 health sciences
Characidae
Gene Expression Regulation, Developmental
Histology
04 agricultural and veterinary sciences
General Medicine
Sertoli cell
Immunohistochemistry
Gonadosomatic Index
medicine.anatomical_structure
040102 fisheries
0401 agriculture, forestry, and fisheries
Female
Subjects
Details
- ISSN :
- 15735168 and 09201742
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Fish Physiology and Biochemistry
- Accession number :
- edsair.doi.dedup.....c127239d3e1ab7b1116e9e8c6085dc36