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The anti-oxidative and anti-inflammatory effects of caffeoyl derivatives from the roots of Aconitum koreanum R. RAYMOND
- Source :
- Biologicalpharmaceutical bulletin. 32(12)
- Publication Year :
- 2009
-
Abstract
- Activity guided fractionation of Aconitum koreanum root extract (RAK), a traditional medicine in Korea, afforded four caffeoyl derivatives, caffeic acid (1), 4,5-dicaffeoylquinic acid (2), 3,5-dicaffeoylquinic acid (3) and 3,5-dicaffeoylquinic acid methyl ester (4). In order to evaluate the anti-oxidative and anti-inflammatory effects of these compounds, their 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities and abilities to inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 were examined. And the protein and mRNA levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated HaCaT cells were also quantified by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). Compounds (1-4) showed potent DPPH radical scavenging and NO inhibitory activities as compared with positive controls (L-ascorbic acid and N(G)-monomethyl-L-arginine (L-NMMA), respectively). Also, these compounds dose-dependently inhibited the expressions of iNOS and COX-2 as well as their mRNA levels.
- Subjects :
- Keratinocytes
Lipopolysaccharides
DPPH
medicine.drug_class
Blotting, Western
Anti-Inflammatory Agents
Cell Culture Techniques
Pharmaceutical Science
Nitric Oxide Synthase Type II
Nitric Oxide
Plant Roots
Anti-inflammatory
Antioxidants
Nitric oxide
chemistry.chemical_compound
Mice
Caffeic Acids
Picrates
Caffeic acid
medicine
Animals
Humans
RNA, Messenger
Aconitum
Pharmacology
biology
Dose-Response Relationship, Drug
Plant Extracts
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Biphenyl Compounds
General Medicine
biology.organism_classification
Nitric oxide synthase
Biphenyl compound
HaCaT
chemistry
Biochemistry
Cyclooxygenase 2
biology.protein
Subjects
Details
- ISSN :
- 13475215
- Volume :
- 32
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Biologicalpharmaceutical bulletin
- Accession number :
- edsair.doi.dedup.....c1085d97d6f983d9b88d2c05f179d38a