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Evolving a Thermostable Terminal Deoxynucleotidyl Transferase
- Source :
- ACS Synthetic Biology. 9:1725-1735
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- Terminal deoxynucleotidyl transferase (TdT) catalyzes template free incorporation of arbitrary nucleotides onto single-stranded DNA. Due to this unique feature, TdT is widely used in biotechnology and clinical applications. One particularly tantalizing use is the synthesis of long
- Subjects :
- 0106 biological sciences
endocrine system
Oligonucleotides
Biomedical Engineering
DNA, Single-Stranded
DNA-Directed DNA Polymerase
Protein Engineering
01 natural sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Catalysis
03 medical and health sciences
chemistry.chemical_compound
DNA Nucleotidylexotransferase
010608 biotechnology
Escherichia coli
Animals
Nucleotide
Amino Acid Sequence
030304 developmental biology
Thermostability
chemistry.chemical_classification
0303 health sciences
Template free
Temperature
General Medicine
Protein engineering
Kinetics
stomatognathic diseases
Terminal deoxynucleotidyl transferase
chemistry
Biochemistry
Mutagenesis
Feature (computer vision)
Cattle
Mutant Proteins
DNA
Plasmids
Subjects
Details
- ISSN :
- 21615063
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- ACS Synthetic Biology
- Accession number :
- edsair.doi.dedup.....c103693f08799c4a1c60f0cd8e4c3fcd
- Full Text :
- https://doi.org/10.1021/acssynbio.0c00078