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IGF2 improves the developmental competency and meiotic structure of oocytes from aged mice
- Source :
- Aging (Albany NY)
- Publication Year :
- 2020
- Publisher :
- Impact Journals, LLC, 2020.
-
Abstract
- Advanced maternal-age is a major factor adversely affecting oocyte quality, consequently worsening pregnancy outcomes. Thus, developing strategies to reduce the developmental defects associated with advanced maternal-age would benefit older mothers. Multiple growth factors involved in female fertility have been extensively studied; however, the age-related impacts of various growth factors remain poorly studied. In the present study, we identified that levels of insulin-like growth factor 2 (IGF2) are significantly reduced in the serum and oocytes of aged mice. We found that adding IGF2 in culture medium promotes oocyte maturation and significantly increases the proportion of blastocysts: from 41% in the untreated control group to 64% (50 nM IGF2) in aged mice (p < 0.05). Additionally, IGF2 supplementation of the culture medium reduced reactive oxygen species production and the incidence of spindle/chromosome defects. IGF2 increases mitochondrial functional activity in oocytes from aged mice: we detected increased ATP levels, elevated fluorescence intensity of mitochondria, higher mitochondrial membrane potentials, and increased overall protein synthesis, as well as increased autophagy activity and decreased apoptosis. Collectively, our findings demonstrate that IGF2 supplementation in culture media improves oocyte developmental competence and reduces meiotic structure defects in oocytes from aged mice.
- Subjects :
- Aging
medicine.medical_treatment
Embryonic Development
Apoptosis
Spindle Apparatus
Mitochondrion
Biology
Andrology
Mice
Meiosis
Insulin-Like Growth Factor II
Autophagy
medicine
Animals
Inner mitochondrial membrane
Cells, Cultured
Membrane Potential, Mitochondrial
chemistry.chemical_classification
Reactive oxygen species
Growth factor
IGF2
Cell Biology
Oocyte
Culture Media
In Vitro Oocyte Maturation Techniques
Mitochondria
Oxidative Stress
medicine.anatomical_structure
chemistry
maternal age
Oocytes
Female
oocyte quality
Reactive Oxygen Species
Research Paper
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....c0dba7033f7705606b7cd0fe5ec48ca4