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IFN-Producing Killer Dendritic Cells Are Antigen-Presenting Cells Endowed with T-Cell Cross-Priming Capacity
- Source :
- Cancer Research. 69:6607-6614
- Publication Year :
- 2009
- Publisher :
- American Association for Cancer Research (AACR), 2009.
-
Abstract
- IFN-producing killer dendritic cells (IKDC) represent a recently discovered cell type in the immune system that possesses a number of functions contributing to innate and adaptive immunity, including production of type 1and 2 IFNs, interleukin (IL)-12, natural killing, and ultimately antigen presentation to naïve T cells. Here, we compared in vitro and in vivo responses of mouse IKDC, conventional dendritic cells (DC), and natural killer (NK) cells to murine cytomegalovirus infection and found distinct functions among these cell subsets. Upon recognition of infected fibroblasts, IKDC, as well as NK, produced high level of IFN-γ, but unlike NK, IKDC simultaneously produced IL-12p40 and up-regulated MHC class II (MHC-II) and costimulatory molecules. Using MHC-II molecule expression as a phenotypic marker to distinguish activated IKDC from activated NK, we further showed that highly purified MHC-II+ IKDC but not NK cross-present MHC class I-restricted antigens derived from MCMV-infected targets to CD8+ T cells in vitro and in vivo. Our findings emphasize the unique nature of IKDC as a killer antigen-presenting cell directly linking innate and adaptive immunity. ©2009 American Association for Cancer Research.<br />link_to_subscribed_fulltext
- Subjects :
- Cytotoxicity, Immunologic
Cancer Research
T-Lymphocytes
T cell
Antigen presentation
Antigen-Presenting Cells
chemical and pharmacologic phenomena
Mice, SCID
Biology
Article
Mice
Interleukin 21
Cross-Priming
Chemicals And Cas Registry Numbers
medicine
Animals
Antigen-presenting cell
Cells, Cultured
Mice, Knockout
Mice, Inbred BALB C
Lymphokine-activated killer cell
Dendritic Cells
Dendritic cell
Natural killer T cell
Interleukin-12
Mice, Inbred C57BL
medicine.anatomical_structure
Oncology
Myeloid Differentiation Factor 88
Immunology
Interleukin 12
Interferons
Subjects
Details
- ISSN :
- 15387445 and 00085472
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Cancer Research
- Accession number :
- edsair.doi.dedup.....c0c5f7b546085d63489a6d80389ecaea