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The Tannat genome: Unravelling its unique characteristics

Authors :
Francisco Carrau
Carina Gaggero
Alberto Ferrarini
C. Da Silva
Alessandra Dal Molin
Massimo Delledonne
Eduardo Boido
Source :
BIO Web of Conferences, Vol 12, p 01016 (2019)
Publication Year :
2019
Publisher :
EDP Sciences, 2019.

Abstract

Tannat (Vitis vinifera) is the most cultivated grapevine variety in Uruguay for the production of high quality red wines. Its berries have unusually high levels of polyphenolic compounds (anthocyanins and tannins), producing wines with intense purple colour and high antioxidant properties. Remarkably, more than 40% of its tannins are galloylated, which determines a greater antioxidant power. Technologies of massive sequencing allow the characterization of genomic variants between different clutivars. The Tannat genome was sequenced with a 134X coverage using the Illumina technology, and was annotated using transcriptomes (RNA-Seq) of different berry tissues. When comparing the genomes of Tannat with Pinot Noir PN40024 (reference genome) we found an expansion of the gene families related to the biosynthesis of polyphenols. A search base on the recently reported epicatechin galloyl transferase (ECGT) from tea leaves determined five putative genes encoding the ECGT in Tannat. Genetic analysis of one of the transcription factor that regulates the anthocyanin synthesis during berry ripening, VvMYBA1, shows the presence of Gret1 retrotransposon in one of the VvMYBA1 alleles in the Tannat clones analysed. This work makes original contributions about the molecular bases of the biosynthesis of anthocyanins and tannins during the development of the flagship grape of Uruguay.

Details

Language :
English
ISSN :
21174458
Volume :
12
Database :
OpenAIRE
Journal :
BIO Web of Conferences
Accession number :
edsair.doi.dedup.....c0c57ca2400b3d81c7d3cd1616099a17