Metabolites of pinacolyl methylphosphonofluoridate (Soman) after enzymatic hydrolysis in vitro

Analytical procedures for the characterization and quantitative estimation of phosphonic acids derived from Soman, based upon paper chromatography and partition between isobutanol-benzene and an aqueous phase, were developed. Utilization of 32P-labeled phosphonic acids permitted the determination of as little as 10 −2 μmole of acid. Application of these methods to the enzymatic hydrolysis products of Soman suggests that the principal, if not the only, metabolite of Soman incubated with rat plasma or liver tissue in vitro is pinacolyl methylphosphonic acid.