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High-throughput sequencing in mutation detection: A new generation of genotoxicity tests?
- Source :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 776:136-143
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- The advent of next generation sequencing (NGS) technology has provided the means to directly analyze the genetic material in primary cells or tissues of any species in a high throughput manner for mutagenic effects of potential genotoxic agents. In principle, direct, genome-wide sequencing of human primary cells and/or tissue biopsies would open up opportunities to identify individuals possibly exposed to mutagenic agents, thereby replacing current risk assessment procedures based on surrogate markers and extrapolations from animal studies. NGS-based tests can also precisely characterize the mutation spectra induced by genotoxic agents, improving our knowledge of their mechanism of action. Thus far, NGS has not been widely employed in genetic toxicology due to the difficulties in measuring low-abundant somatic mutations. Here, we review different strategies to employ NGS for the detection of somatic mutations in a cost-effective manner and discuss the potential applicability of these methods in testing the mutagenicity of genotoxic agents.
- Subjects :
- Genetics
Mutation Spectra
Somatic cell
DNA damage
Health, Toxicology and Mutagenesis
DNA Mutational Analysis
High-Throughput Nucleotide Sequencing
Genome-wide association study
Biology
medicine.disease_cause
Article
DNA sequencing
Mutation
Mutation (genetic algorithm)
medicine
Animals
Humans
Molecular Biology
Genotoxicity
DNA Damage
Genome-Wide Association Study
Genetic Toxicology
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 776
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- Accession number :
- edsair.doi.dedup.....c08a32ce13e5495e8ca81a1b95c49135
- Full Text :
- https://doi.org/10.1016/j.mrfmmm.2015.03.014