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High-throughput sequencing in mutation detection: A new generation of genotoxicity tests?

Authors :
Ryan R. White
Alexander Y. Maslov
Wilber Quispe-Tintaya
Tatyana Gorbacheva
Jan Vijg
Source :
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 776:136-143
Publication Year :
2015
Publisher :
Elsevier BV, 2015.

Abstract

The advent of next generation sequencing (NGS) technology has provided the means to directly analyze the genetic material in primary cells or tissues of any species in a high throughput manner for mutagenic effects of potential genotoxic agents. In principle, direct, genome-wide sequencing of human primary cells and/or tissue biopsies would open up opportunities to identify individuals possibly exposed to mutagenic agents, thereby replacing current risk assessment procedures based on surrogate markers and extrapolations from animal studies. NGS-based tests can also precisely characterize the mutation spectra induced by genotoxic agents, improving our knowledge of their mechanism of action. Thus far, NGS has not been widely employed in genetic toxicology due to the difficulties in measuring low-abundant somatic mutations. Here, we review different strategies to employ NGS for the detection of somatic mutations in a cost-effective manner and discuss the potential applicability of these methods in testing the mutagenicity of genotoxic agents.

Details

ISSN :
00275107
Volume :
776
Database :
OpenAIRE
Journal :
Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
Accession number :
edsair.doi.dedup.....c08a32ce13e5495e8ca81a1b95c49135
Full Text :
https://doi.org/10.1016/j.mrfmmm.2015.03.014