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Following replicative DNA synthesis by time-resolved X-ray crystallography
- Source :
- Nature communications, vol 12, iss 1, Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
- Publication Year :
- 2021
- Publisher :
- eScholarship, University of California, 2021.
-
Abstract
- The mechanism of DNA synthesis has been inferred from static structures, but the absence of temporal information raises longstanding questions about the order of events in one of life’s most central processes. Here we follow the reaction pathway of a replicative DNA polymerase using time-resolved X-ray crystallography to elucidate the order and transition between intermediates. In contrast to the canonical model, the structural changes observed in the time-lapsed images reveal a catalytic cycle in which translocation precedes catalysis. The translocation step appears to follow a push-pull mechanism where the O-O1 loop of the finger subdomain acts as a pawl to facilitate unidirectional movement along the template with conserved tyrosine residues 714 and 719 functioning as tandem gatekeepers of DNA synthesis. The structures capture the precise order of critical events that may be a general feature of enzymatic catalysis among replicative DNA polymerases.
- Subjects :
- DNA, Bacterial
0301 basic medicine
DNA Replication
Time Factors
DNA polymerase
Science
1.1 Normal biological development and functioning
General Physics and Astronomy
Crystallography, X-Ray
010402 general chemistry
01 natural sciences
General Biochemistry, Genetics and Molecular Biology
Enzyme catalysis
03 medical and health sciences
chemistry.chemical_compound
Genetic
Underpinning research
Models
Escherichia coli
Tyrosine
Multidisciplinary
Crystallography
Models, Genetic
DNA synthesis
biology
Chemistry
Escherichia coli Proteins
DNA replication
Bacterial
General Chemistry
DNA
DNA Polymerase I
0104 chemical sciences
030104 developmental biology
Order (biology)
Catalytic cycle
Biophysics
biology.protein
X-Ray
HIV/AIDS
Nucleic Acid Conformation
Generic health relevance
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Nature communications, vol 12, iss 1, Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021)
- Accession number :
- edsair.doi.dedup.....c06189fcd0e217f2794a62a16c68ab7c