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Fluorescent Anti-MUC5AC Brightly Targets Pancreatic Cancer in a Patient-derived Orthotopic Xenograft
- Source :
- In Vivo, In vivo (Athens, Greece), vol 36, iss 1
- Publication Year :
- 2021
- Publisher :
- Anticancer Research USA Inc., 2021.
-
Abstract
- Background: Overexpression of mucin-5AC (MUC5AC) makes it a targetable biomarker in pancreatic cancer. The present study evaluated tumor targeting with a MUC5AC antibody conjugated to a near-infrared dye in a patient-derived orthotopic xenograft (PDOX) mouse model. Materials and Methods: MUC5AC monoclonal antibody was conjugated to the near-infrared dye IRDye800CW to synthesize MUC5AC-IR800. PDOX models were established by implanting a high-MUC5AC-expressing patient-derived pancreatic tumor on the pancreas of nude mice. After 4 weeks of PDOX tumor growth, mice were imaged after receiving MUC5AC-IR800 (75 μg) intravenously. Results: In the PDOX models, MUC5AC-IR800 selectively and brightly targeted the pancreatic tumor (tumor to background ratio: 2.46±0.465). Conclusion: MUC5AC-IR800 provides distinct visualization of pancreatic tumors. MUC5AC-IR800 may be used clinically in the future to improve pancreatic cancer resection. This novel fluorescent probe is also promising for targeting of pre-malignant pancreatic lesions with subsequent resection under fluorescence guidance.
- Subjects :
- tumor-specific imaging
Cancer Research
Nude
Clinical Sciences
Mice, Nude
digestive system
General Biochemistry, Genetics and Molecular Biology
fluorescence guided surgery
PDOX
Pancreatic Cancer
Mice
Rare Diseases
fluids and secretions
mucin
antibody
2.1 Biological and endogenous factors
Animals
Humans
Oncology & Carcinogenesis
Aetiology
Cancer
Fluorescent Dyes
Pharmacology
respiratory system
MUC5AC
Xenograft Model Antitumor Assays
Pancreatic Neoplasms
Orphan Drug
Good Health and Well Being
Heterografts
sense organs
Digestive Diseases
Biotechnology
Research Article
Subjects
Details
- ISSN :
- 17917549 and 0258851X
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- In Vivo
- Accession number :
- edsair.doi.dedup.....c05b113c89a4c348ce4b63739468f5c9