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Diversity and enzymatic potentialities of Bacillus sp. strains isolated from a polluted freshwater ecosystem in Cuba
- Source :
- World Journal of Microbiology and Biotechnology, Vol. 34, p. 28 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Genotypic and phenotypic characterization of Bacillus spp. from polluted freshwater has been poorly addressed. The objective of this research was to determine the diversity and enzymatic potentialities of Bacillus spp. strains isolated from the Almendares River. Bacilli strains from a polluted river were characterized by considering the production of extracellular enzymes using API ZYM. 14 strains were selected and identified using 16S rRNA, gyrB and aroE genes. Genotypic diversity of the Bacillus spp. strains was evaluated using pulsed field gel electrophoresis. Furthermore, the presence of genetic determinants of potential virulence toxins of the Bacillus cereus group and proteinaceous crystal inclusions of Bacillus thuringiensis was determined. 10 strains were identified as B. thuringiensis, two as Bacillus megaterium, one as Bacillus pumilus and one as Bacillus subtilis. Most strains produced proteases, amylases, phosphatases, esterases, aminopeptidases and glucanases, which reflect the abundance of biopolymeric matter in Almendares River. Comparison of the typing results revealed a spatio-temporal distribution among B. thuringiensis strains along the river. The results of the present study highlight the genotypic and phenotypic diversity of Bacillus spp. strains from a polluted river, which contributes to the knowledge of genetic diversity of Bacilli from tropical polluted freshwater ecosystems.
- Subjects :
- DNA, Bacterial
0301 basic medicine
Bacilli
Genotype
Physiology
Bacterial Toxins
030106 microbiology
Bacillus thuringiensis
Bacillus cereus
Bacillus
Fresh Water
Bacillus subtilis
Applied Microbiology and Biotechnology
Microbiology
03 medical and health sciences
Freshwater
Bacterial Proteins
Rivers
Species Specificity
RNA, Ribosomal, 16S
Enzyme activity
Polluted river
Ecosystem
Phylogeny
Enzyme Assays
Bacillus megaterium
Genetic diversity
Virulence
biology
Bacillus pumilus
Water Pollution
fungi
Cuba
Q Science (General)
Biodiversity
General Medicine
biology.organism_classification
16S ribosomal RNA
Electrophoresis, Gel, Pulsed-Field
030104 developmental biology
DNA Gyrase
Genes, Bacterial
bacteria
Water Microbiology
Bacillus spp
Biotechnology
Subjects
Details
- ISSN :
- 15730972 and 09593993
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- World Journal of Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....c0363528478866cd605f8741344dca59