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Adhesion/growth-regulatory galectins tested in combination: evidence for formation of hybrids as heterodimers
- Source :
- Biochemical Journal, 475(5), 1003-1018. Portland Press Ltd.
- Publication Year :
- 2018
- Publisher :
- Portland Press Ltd., 2018.
-
Abstract
- The delineation of the physiological significance of protein (lectin)–glycan recognition and the structural analysis of individual lectins have directed our attention to studying them in combination. In this report, we tested the hypothesis of hybrid formation by using binary mixtures of homodimeric galectin-1 and -7 as well as a proteolytically truncated version of chimera-type galectin-3. Initial supportive evidence is provided by affinity chromatography using resin-presented galectin-7. Intriguingly, the extent of cell binding by cross-linking of surface counter-receptor increased significantly for monomeric galectin-3 form by the presence of galectin-1 or -7. Pulsed-field gradient NMR (nuclear magnetic resonance) diffusion measurements on these galectin mixtures indicated formation of heterodimers as opposed to larger oligomers. 15N-1H heteronuclear single quantum coherence NMR spectroscopy and molecular dynamics simulations allowed us to delineate how different galectins interact in the heterodimer. The possibility of domain exchange between galectins introduces a new concept for understanding the spectrum of their functionality, particularly when these effector molecules are spatially and temporally co-expressed as found in vivo.
- Subjects :
- 0301 basic medicine
Galectin 1
Galectin 3
Galectins
DIFFUSION MEASUREMENTS
PANCREATIC-CARCINOMA MODEL
Biochemistry
CHEMICAL-SHIFT ASSIGNMENTS
03 medical and health sciences
Molecular dynamics
0302 clinical medicine
Affinity chromatography
Tumor Cells, Cultured
HUMAN NEUROBLASTOMA-CELLS
otorhinolaryngologic diseases
Humans
Molecule
Molecular Biology
Cell Proliferation
Galectin
Binding Sites
biology
Effector
Chemistry
Proteins
Lectin
Blood Proteins
IN-VITRO
Cell Biology
Nuclear magnetic resonance spectroscopy
stomatognathic diseases
030104 developmental biology
MOLECULAR-DYNAMICS
P53-INDUCED GENE-1
030220 oncology & carcinogenesis
biology.protein
Biophysics
Protein Multimerization
N-15 BACKBONE
CARBOHYDRATE-RECOGNITION DOMAIN
Cell Adhesion Molecules
Heteronuclear single quantum coherence spectroscopy
CHIMERA-TYPE GALECTIN-3
Subjects
Details
- ISSN :
- 14708728 and 02646021
- Volume :
- 475
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....c032e5b26e9427b67d59c94d88330622