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Cosecretion of Prolactin and Growth Hormone by Dispersed Pituitary Cells of the Adult Bullfrog, Rana catesbeiana
- Source :
- General and Comparative Endocrinology. 122:10-16
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- The coexistence of prolactin (PRL) and growth hormone (GH) was previously demonstrated in newly hatched bullfrog (Rana catesbeiana) tadpoles, whereas in adult bullfrogs, there were no cells containing both PRL and GH. However, a cell blot assay with enzymatically dispersed adult pituitary cells demonstrated the existence of cells secreting both PRL and GH. The number of cells secreting both PRL and GH was reduced by a protein synthesis inhibitor, cycloheximide, but not by an RNA synthesis inhibitor, actinomycin D. In situ hybridization and immunostaining of intact pituitary glands revealed the existence of GH mRNA in some of the PRL-immunoreactive cells and of PRL mRNA in some of the GH-immunoreactive cells. We propose that dispersion of the pituitary cells triggered the translation of GH mRNA in the PRL cells and/or of PRL mRNA in the GH cells.
- Subjects :
- endocrine system
Pituitary gland
medicine.medical_specialty
endocrine system diseases
Gene Expression
In situ hybridization
Biology
Cycloheximide
Immunoenzyme Techniques
chemistry.chemical_compound
Endocrinology
Bullfrog
Internal medicine
medicine
Animals
RNA, Messenger
In Situ Hybridization
Nucleic Acid Synthesis Inhibitors
Protein Synthesis Inhibitors
Rana catesbeiana
Prolactin
Growth hormone secretion
Blot
medicine.anatomical_structure
chemistry
Growth Hormone
Pituitary Gland
Dactinomycin
Female
Animal Science and Zoology
hormones, hormone substitutes, and hormone antagonists
Immunostaining
Subjects
Details
- ISSN :
- 00166480
- Volume :
- 122
- Database :
- OpenAIRE
- Journal :
- General and Comparative Endocrinology
- Accession number :
- edsair.doi.dedup.....c03032c27846a6773bc3c48517a87a94