Additional file 6 of Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis

Additional file 6 Fig. S4 (Relates to Fig. 4) (A) Single-cell transcriptomes from porcine undifferentiated spermatogonia, differentiating spermatogonia, Leptotene and Zygotene spermatocytes were used for cell trajectories ordered in pseudotime (right) and cells colored according to cell cluster (left). (B) Heatmaps show the top 50 variable genes across pseudotime from porcine undifferentiated spermatogonia, differentiating spermatogonia, Leptotene and Zygotene spermatocytes (scaled expression according to legend). (C) Visualization of marker gene expression across the differentiation states of spermatogonia in the violin plot. Unbiased cell clusters are distinguished by color according to the key. (D) Expression patterns of new defined marker genes for undifferentiated and differentiating spermatogonia visualized in t-SNE plots for all cell types.