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Novel oligosaccharides with the sialyl-Lea structure in human milk
- Source :
- Biochemistry. 30:2869-2876
- Publication Year :
- 1991
- Publisher :
- American Chemical Society (ACS), 1991.
-
Abstract
- We have isolated four novel oligosaccharides with the sialyl-Lea structure from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. The results of structural analyses, i.e., 500-MHz 1H NMR spectroscopy, fast atom bombardment mass spectrometry, and binding to specific anticarbohydrate antibodies, are consistent with the following structures. (formula; see text)
- Subjects :
- Magnetic Resonance Spectroscopy
medicine.drug_class
Molecular Sequence Data
Oligosaccharides
Spectrometry, Mass, Fast Atom Bombardment
Mass spectrometry
Monoclonal antibody
Biochemistry
Chromatography, Affinity
Epitope
Affinity chromatography
Carbohydrate Conformation
medicine
Humans
Chromatography, High Pressure Liquid
chemistry.chemical_classification
Chromatography
Milk, Human
biology
medicine.diagnostic_test
Chemistry
Antibodies, Monoclonal
Oligosaccharide
Fast atom bombardment
Carbohydrate Sequence
Immunoassay
biology.protein
Female
Antibody
Subjects
Details
- ISSN :
- 15204995 and 00062960
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....bffe3d03183b6d9e63b2726bd4c4d82d