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Extraribosomal function of the acidic ribosomal P1-protein YP1alpha from Saccharomyces cerevisiae
- Source :
- Acta biochimica Polonica. 46(4)
- Publication Year :
- 2000
-
Abstract
- The yeast acidic ribosomal P-proteins YP1alpha, YP1beta, YP2alpha and YP2beta were studied for a possible transactivation potential beside their ribosomal function. The fusions of P-proteins with the GAL4 DNA-binding domain were assayed toward their transcriptional activity with the aid of reporter genes in yeast. Two of the P-proteins, YP1alpha and YP1beta, exhibited transactivation potential, however, only YP1alpha can be regarded as a potent transactivator. This protein was able to transactivate a reporter gene associated with two distinct promoter systems, GAL1 or CYC1. Additionally, truncated proteins of YP1alpha and YP1beta were analyzed. The N-terminal part of YP1alpha fused to GAL4-BD showed transactivation potential but the C-terminal part did not. Our results suggest a putative extraribosomal function for these ribosomal proteins which consequently may be classified as "moonlighting" proteins.
- Subjects :
- Ribosomal Proteins
Transcriptional Activation
Recombinant Fusion Proteins
Saccharomyces cerevisiae
Genes, Fungal
General Biochemistry, Genetics and Molecular Biology
Fungal Proteins
Transactivation
5S ribosomal RNA
Ribosomal protein
Genes, Reporter
30S
Eukaryotic Small Ribosomal Subunit
Promoter Regions, Genetic
DNA Primers
Sequence Deletion
biology
Base Sequence
Chemistry
Eukaryotic Large Ribosomal Subunit
Ribosomal RNA
biology.organism_classification
Phosphoproteins
Molecular biology
Cell biology
Lac Operon
Ribosomes
Subjects
Details
- ISSN :
- 0001527X
- Volume :
- 46
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Acta biochimica Polonica
- Accession number :
- edsair.doi.dedup.....bfcc3dcf2d29fe633ee2458a8a63bd63