A method to analyze, sort, and retain viability of obligate anaerobic microorganisms from complex microbial communities

Authors :: Brian Wadey
Matthew J. Crow
Anne W. Thompson
Christina E. Arens
Nicholas Elliott
David A. Stahl
Ger van den Engh
Timothy W. Petersen
Sergey Stolyar
Serdar Turkarslan
Nitin S. Baliga
Source :: Journal of Microbiological Methods. :74-77
Publisher :: The Authors. Published by Elsevier B.V.
Abstract: A high speed flow cytometric cell sorter was modified to maintain a controlled anaerobic environment. This technology enabled coupling of the precise high-throughput analytical and cell separation capabilities of flow cytometry to the assessment of cell viability of evolved lineages of obligate anaerobic organisms from cocultures.

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