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Gastrin-releasing peptide-like immunoreactivity is present in human maternal and fetal placental membranes
Gastrin-releasing peptide-like immunoreactivity is present in human maternal and fetal placental membranes
- Source :
- The Journal of Clinical Endocrinology & Metabolism. 81:3766-3773
- Publication Year :
- 1996
- Publisher :
- The Endocrine Society, 1996.
-
Abstract
- Extracts of human term amnion, placenta, and chorion/decidual tissue (n = 5) contained gastrin-releasing peptide-like immunoreactivity (GRPLI) in amounts of 4.7 +/- 2.9 (pmol/g wet wt; mean +/- SEM), 3.6 +/- 1.1 and 2.9 +/- 1.5, respectively. Using C-terminally directed antisera and gel filtration chromatography and reverse-phase high-performance liquid chromatography (HPLC), each tissue contained molecular forms consistent with the presence of GRP1-27 and GRP18-27 but also contained larger amounts of two GRPLI peaks, which apparently are novel GRP-like peptides. In contrast, tissue extracts of human fetal lung contained only GRP1-27, GRP14-27, and GRP18-27. Using RT-PCR and specific GRP primers and probes, messenger RNA encoding for GRP was readily demonstrable from 6-weeks gestation throughout pregnancy to term in full-thickness membranes, placental villi, and decidua. Positive immunohistochemical staining for GRP occurred in extravillous trophoblasts in decidual septa and fetal membranes, cytotrophoblasts, syncytiotrophoblast, and certain stromal cells in placental villi and amniotic epithelium. GRPLI and GRP messenger RNA were present from the earliest dates examined (6-9 weeks) throughout pregnancy to term. Given the proven trophic nature of GRP and related peptides, these peptides may play important roles in maternal, placental, and fetal development during human pregnancy.
- Subjects :
- medicine.medical_specialty
Placenta
Endocrinology, Diabetes and Metabolism
Clinical Biochemistry
Radioimmunoassay
Biology
Polymerase Chain Reaction
Biochemistry
Endocrinology
Pregnancy
Internal medicine
Gastrin-releasing peptide
Decidua
medicine
Humans
Amnion
RNA, Messenger
Chromatography, High Pressure Liquid
DNA Primers
Fetus
Biochemistry (medical)
RNA-Directed DNA Polymerase
Chorion
Immunohistochemistry
Peptide Fragments
Epithelium
medicine.anatomical_structure
Membrane
Gastrin-Releasing Peptide
embryonic structures
Chromatography, Gel
Chorionic villi
Bombesin
Female
Peptides
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 81
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi.dedup.....bf8f297adbb6db69c87972aa171f0287
- Full Text :
- https://doi.org/10.1210/jcem.81.10.8855836