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Marinilactibacillus piezotolerans sp. nov., a novel marine lactic acid bacterium isolated from deep sub-seafloor sediment of the Nankai Trough
- Source :
- International Journal of Systematic and Evolutionary Microbiology, International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2005, Vol. 55, N. 1, P. 345-351
- Publication Year :
- 2005
-
Abstract
- A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20T) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaCl concentration range for growth was 0–120 g l−1, with the optimum at 10–20 g l−1. The temperature range for growth at pH 7·0 was 4–50 °C, with the optimum at 37–40 °C. The optimum pH for growth was 7·0–8·0. The optimum pressure for growth was 0·1 MPa with tolerance up to 30 MPa. The main cellular phospholipids were phosphatidylglycerols (25 %), diphosphatidylglycerols (34 %) and a group of compounds tentatively identified as ammonium-containing phosphatidylserines (32 %); phosphatidylethanolamines (9 %) were minor components. The fatty acid composition was dominated by side chains of 16 : 0, 14 : 0 and 16 : 1. The G+C content of the genomic DNA was 42 mol%. On the basis of 16S rRNA gene sequence analysis and the secondary structure of the V6 region, this organism was found to belong to the genus Marinilactibacillus and was closely related to Marinilactibacillus psychrotolerans M13-2T (99 %), Marinilactibacillus sp. strain MJYP.25.24 (99 %) and Alkalibacterium olivapovliticus strain ww2-SN4C (97 %). Despite the high similarity between their 16S rRNA gene sequences (99 %), the DNA–DNA hybridization levels were less than 20 %. On the basis of physiological and genetic characteristics, it is proposed that this organism be classified as a novel species, Marinilactibacillus piezotolerans sp. nov. The type strain is LT20T (=DSM 16108T=JCM 12337T).
- Subjects :
- DNA, Bacterial
Geologic Sediments
Sequence analysis
Deep sub seafloor sediment
Thermophilic bacteria
Molecular Sequence Data
Hydrostatic pressure
550 - Earth sciences
Gram-Positive Asporogenous Rods
Biochemistry
DNA, Ribosomal
Microbiology
03 medical and health sciences
Japan
RNA, Ribosomal, 16S
Hydrostatic Pressure
Seawater
Lactic Acid
Food science
Phylogeny
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Thermophile
Genes, rRNA
Sequence Analysis, DNA
General Medicine
Ribosomal RNA
16S ribosomal RNA
biology.organism_classification
Bacterial Typing Techniques
genomic DNA
Phenotype
Bacteria
Mesophile
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology, International journal of systematic and evolutionary microbiology (1466-5026) (Society for General Microbiology), 2005, Vol. 55, N. 1, P. 345-351
- Accession number :
- edsair.doi.dedup.....bf8d21982a84b944cd482c94dc1018c9